Porcine isogloboside 3 synthase protein, cDNA, genomic organization, and regulatory region
The present invention provides porcine isogloboside 3 (iGb3) synthase protein, cDNA, and genomic DNA regulatory sequence. The present also invention includes porcine animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which lack expression of functional iGb3 synthase. Such animals, tissues, organs and cells can be used in research and in medical therapy, including xenotransplantation. In addition, methods are provided to prepare organs, tissues, and cells lacking the porcine iGb 3 synthase gene for use in xenotransplantation.
1. An isolated cDNA sequence encoding a porcine IsoGloboside 3 (iGb3) synthase peptide, wherein the sequence comprises SEQ ID NO: 1.
2. A nucleic acid construct comprising a cDNA sequence encoding a porcine IsoGloboside 3 (iGb3) synthase protein, wherein the cDNA sequence comprises SEQ ID NO: 1.
3. The construct of claim 2 further comprising a promoter.
4. The construct of claim 2 further comprising a selectable marker.
5. The construct of claim 4 wherein the selectable marker is green fluorescent protein.
6. An isolated transfected cell comprising the construct of claim 2 .
7. An isolated nucleotide sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, and SEQ ID NO: 13.
8. A nucleic acid construct comprising at least 500 contiguous nucleic acids selected from the group consisting of SEQ ID NO: 6 and SEQ ID NO: 1.
9. The nucleic acid construct of claim 8 , wherein the construct comprises at least 1000 nucleic acids.
10. A nucleic acid construct comprising a nucleic acid sequence encoding at least 160 contiguous amino acids of SEQ ID NO: 2.