Caterpiller gene family
View Patent ↗The present invention relates to a new family of structurally and functionally related nucleic acids and proteins, designed the CATERPILLER family, which is characterized by landmark structural motifs including a nucleotide binding domain and leucine-rich repeat domains.
1. An isolated nucleic acid encoding a CATERPILLER 11.3 polypeptide comprising a nucleotide sequence selected from the group consisting of:
(a) the nucleotide sequence of SEQ ID NO:17 or SEQ ID NO:19;
(b) a nucleotide sequence that differs from the nucleotide sequences of (a) above due to the degeneracy of the genetic code.
2. An isolated cell comprising the isolated nucleic acid of claim 1 .
3. A method of identifying a compound that binds to a CATERPILLER 11.3 polypeptide encoded by the nucleic acid of claim 1 comprising:
contacting the polypeptide with a test compound under conditions whereby binding between the polypeptide and the test compound can be detected; and
detecting binding between the polypeptide and the test compound.
4. A method of identifying a compound that modulates the activity of a CATERPILLER 11.3 polypeptide encoded by the nucleic acid of claim 1 comprising:
contacting the polypeptide with a test compound under conditions whereby modulation of the activity of the polypeptide can be detected; and
detecting modulation of the activity of the polypeptide, wherein the activity of the polypeptide that is measured is inhibition of Myd88-induced NF-κB induction and/or NIK-induced NF-κB induction.
5. The method of claim 3 , wherein the method is carried out in a cell comprising the polypeptide.
6. The method of claim 5 , wherein the cell comprises an isolated nucleic acid comprising a nucleotide sequence encoding the polypeptide.
7. The method of claim 6 , wherein the cell is stably transformed with the isolated nucleic acid.
8. The method of claim 3 , wherein the method is carried out as a cell-free assay.
9. The method of claim 3 , wherein the method is carried out in a transgenic non-human mammal comprising an isolated nucleic acid comprising a nucleotide sequence encoding the polypeptide.
10. The isolated nucleic acid of claim 1 encoding the polypeptide of SEQ ID NO:18.
11. The isolated nucleic acid of claim 1 encoding the polypeptide of SEQ ID NO:20.