Methods and compositions for diagnosing hepatocellular carcinoma
Methods for the diagnosis of hepatocellular carcinoma (HCC) are set forth. Improved assay methods and scanning methods are included that employ non-cell-associated and cell-associated HCC related proteins. Such methods are based upon the discovery of genes that were up-regulated in diseased versus normal tissue as well as in HCC tissue when compared to the tissue of patients with other ailments.
1. A method of detecting the presence of HCC in a mammal comprising:
a) obtaining a biological sample from the mammal;
b) assaying the sample to quantify a non-cell-associated HCC related protein;
c) comparing the quantity of the non-cell-associated HCC related protein to a control level obtained from a sample without HCC, wherein the non-cell-associated HCC related protein comprises phospholipase A2 group XIII (PLA2G13); and
d) detecting the presence of HCC in said mammal if PLA2G13 is elevated in step c) above.
2. The method of claim 1 wherein assaying the sample is selected from the group consisting of using an enzyme linked immunosorbent assay (ELISA) and competition assays using monoclonal, polyclonal, or a combination of monoclonal and polyclonal antibodies.
3. The method of claim 2 wherein the polyclonal antibodies include those that bind PLA2G13.