Anti-PSGL-1 antibodies
Immunoglobulin chains or antibodies having light or heavy chain complementarity determining regions of antibodies that bind to P-Selectin Glycoprotein Ligand-1. Also disclosed are methods of inducing death of an activated T-cell and of modulating a T cell-mediated immune response in a subject.
1. An isolated antibody that binds specifically to human P-Selectin Glycoprotein Ligand 1 (PSGL-1), comprising a light chain comprising SEQ ID NOs: 1-3 and a heavy chain comprising SEQ ID NOs: 4-6.
2. An isolated antibody that binds specifically to human P-Selectin Glycoprotein Ligand 1 (PSGL-1), wherein the antibody comprises (i) a light chain comprising a variable region having the amino acid sequence of SEQ ID NO: 25 linked to a human kappa light chain constant region, and (ii) a heavy chain comprising a variable region having the amino acid sequence of SEQ ID NO: 26 linked to a human IgG4 heavy chain constant region.
3. A composition comprising the antibody according to claim 2 .
4. The antibody according to claim 1 , wherein the antibody is a chimeric antibody.
5. The antibody according to claim 1 , wherein the antibody is a humanized antibody.
6. A composition comprising the isolated antibody according to claim 1 .
7. An isolated antibody that binds specifically to human P-Selectin Glycoprotein Ligand 1 (PSGL-1), selected from the group consisting of
(a) an antibody comprising a light chain comprising SEQ ID NO: 25;
(b) an antibody comprising a heavy chain comprising SEQ ID NO: 26; and
(c) an antibody comprising a light chain comprising SEQ ID NO: 25 and a heavy chain comprising SEQ ID NO: 26.
8. A composition comprising the isolated antibody according to claim 7 .
9. The isolated antibody according to claim 7 , wherein the antibody comprises a light chain comprising SEQ ID NO: 25 and a heavy chain comprising SEQ ID NO: 26.
10. A composition comprising the isolated antibody according to claim 9 .
11. The isolated antibody according to claim 7 , wherein the antibody comprises a heavy chain constant region of human IgG1.
12. The isolated antibody according to claim 7 , wherein the antibody comprises a heavy chain constant region of human IgG2.
13. The isolated antibody according to claim 7 , wherein the antibody comprises a heavy chain constant region of human IgG4.
14. The isolated antibody according to claim 9 , wherein the heavy chain further comprises a human heavy chain constant region of human IgG4.
15. A composition comprising the isolated antibody according to claim 14 .