Preparation and application of transhintotalphenolic acid
A process for preparing tanshintotalphenolic acid and the use of the product are disclosed. The process comprises: tanshin is hot-extracted with water, the extract is separated and refined by polyamide column and macroporous adsorption resin column, and lyophilized to obtain tanshintotalphenolic acid. The yield of the final end product is more than 4 percent based on the amount of crude drug and the content of totalphenolic acid is more than 80 percent. The tanshintotalphenolic acid obtained can be used as the medicine for preventing and treating cerebrovascular and cardiovascular diseases and so on.
1. A method for the preparation of total salvianolic acid comprising the steps of:
(a) extracting Danshen with water to produce a Danshen extract, acidifying the extract and filtering the extract;
(b) applying the Danshen extract to a polyamide column, washing the polyamide column with water until neutral, discarding the washings, and collecting fractions eluted from the polyamide column with a weak basic aqueous solution;
(c) acidifying the fractions eluted from the polyamide column, applying the fractions to a macroporous adsorption resin column, washing the column with water to achieve neutrality, discarding the washings, and collecting eluate from the macroporous adsorption resin column with a hydrous or anhydrous lower alcohol, wherein the eluate is further optionally concentrated and dried.
2. The method of claim 1 , wherein the step of extracting Danshen with water is repeated 2-4 times for 0.5-2 hours each time.
3. The method of claim 1 , wherein the step of extracting Danshen with water is carried out at 60-100° C.
4. The method of claim 1 , wherein the step of extracting Danshen with water is carried out at 90-100° C.
5. The method of claim 1 , wherein the pH of the Danshen extract is adjusted to below 4, and the pH of the fractions eluted from the polyamide column adjusted to below 4.
6. The method of claim 1 , wherein the pH of the Danshen extract is adjusted to below 2, and the pH of the fractions eluted from the polyamide column is adjusted with hydrochloric acid or sulfuric acid to a value below 2.
7. The method of claim 1 , wherein the fractions eluted from the polyamide column are eluted with 0.01-2% of aqueous weak basic solution.
8. The method of claim 1 , wherein the fractions eluted from the polyamide column are eluted with 0.08-0.5% of an aqueous solution of sodium hydrogen carbonate.
9. The method of claim 1 , wherein the macroporous adsorption resin is a type of styrene.
10. The method of claim 1 , wherein the hydrous or anhydrous lower alcohol is a lower C 1 -C 5 alcohol.
11. The method of claim 1 , wherein the hydrous lower alcohol is an aqueous ethanol with a concentration of 40-95%.
12. The method of claim 10 , wherein the concentration of the lower C 1 -C 5 alcohol is 60-95%.
13. The method of claim 11 , wherein the concentration of the aqueous ethanol is 95%.
14. The method of claim 11 , wherein the aqueous ethanol has a concentration of 60-95%.