Method of electrically detecting biomolecule
Provided is a method of sensing biomolecules using a bioFET, the method including: forming a layer including Au on a gate of the bioFET; forming a probe immobilized on a substrate separated from the gate by a predetermined distance, and a biomolecule having a thiol group (—SH) which is incompletely bonded to the probe; reacting the probe with a sample including a target molecule; and measuring a current flowing in a channel region between a source and a drain of the bioFET.
1. A method of sensing a target nucleic acid in an electrolyte using a bio field effect transistor (FET), the method comprising:
(a) forming a layer comprising Au on a gate of a bio field effect transistor (bioFET);
(b) bonding a nucleic acid having a thiol group (—SH) to a probe nucleic acid immobilized on a substrate separated from the gate by a predetermined distance, wherein the nucleic acid having a thiol group is incompletely bonded to the probe nucleic acid;
(c) contacting the probe nucleic acid with a sample including a target nucleic acid,
wherein a bonding force between the probe nucleic acid and the target nucleic acid is greater than a bonding force between the probe nucleic acid and the nucleic acid having a thiol group;
(d) separating the nucleic acid having a thiol group from the probe nucleic acid by bonding the target nucleic acid to the probe;
(e) bonding the thiol group of the nucleic acid having a thiol group with Au on the gate and
(f) measuring a current flowing in a channel region between a source and a drain of the bioFET.
2. The method of claim 1 , wherein the probe nucleic acid or the target nucleic acid is DNA or RNA.
3. The method of claim 1 , wherein the nucleic acid having a thiol group is DNA or RNA.