Methods and compositions for the treatment and prevention of HIV infection using TRIM5α
The invention provides novel TRIM polypeptides, proteins, and nucleic acid molecules. In addition to isolated, full-length TRIM proteins, the invention further provides isolated TRIM fusion proteins, antigenic peptides and anti-TRIM antibodies. The invention also provides TRIM nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which an TRIM gene has been introduced or disrupted. The present invention also provides methods and compositions for the diagnosis and treatment of viral infection and/or replication, e.g., HIV infection. The invention further provides methods for identifying a compound capable of treating or preventing viral infection and/or replication, e.g., HIV infection and AIDS. In addition, the invention provides a method for treating a subject having a viral infection and/or replication, e.g., HIV infection using the modulators of the invention.
1. An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1, or the full-length complement thereof.
2. An isolated nucleic acid molecule consisting of the nucleotide sequence set forth in SEQ ID NO:1, or the full-length complement thereof.
3. An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, or the full-length complement thereof.
4. An isolated nucleic acid molecule which encodes a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:2, or the full-length complement thereof.
5. An isolated nucleic acid molecule comprising a nucleotide sequence at least 95% identical to the nucleotide sequence of SEQ ID NO:1, wherein the nucleotide sequence encodes a TRIM5α polypeptide, or the full-length complement thereof.
6. An isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:2, or the full-length complement thereof.
7. A vector comprising the nucleic acid molecule of any one of claims 1 - 5 and 6 .
8. The vector of claim 7 , which is an expression vector.
9. The vector of claim 7 , which is a viral vector.
10. An isolated host cell containing the vector of claim 8 or 9 .
11. A method for producing a Trim5α protein comprising culturing the host cell of claim 10 in a suitable medium until said protein is produced.
12. The method of claim 11 , further comprising isolating said protein from the medium or the host cell.