Chimeric CMP-Ang1 molecule
The present application describes a fusion molecule that includes coiled coil domain, which renders the molecule soluble and provides potent activity.
1. A coiled coil chimeric molecule comprising a coiled-coil domain of cartilage matrix protein (CMP) linked to a Tie2 receptor binding domain which is a fibrinogen-like domain of angiopoietin-1.
2. A soluble biologically active multimer comprising the coiled coil chimeric molecule according to claim 1 .
3. The multimer according to claim 2 , which is a dimer, trimer, tetramer, pentamer, hexamer, heptamer, octamer, nanomer or decamer.
4. A method of promoting endothelial cell migration in vitro comprising contacting the coiled coil chimeric molecule of claim 1 , to a population of endothelial cells that express Tie2 receptors, which results in cell migration.