Nontypeable
The invention relates to a mutation within the sap operon of an avirulent clone of a nontypeable strain of Haemophilus influenzae (NTHi). The invention also relates to the NTHi sap operon genes and the polypeptides encoded by these polynucleotide sequences. The invention also relates to a novel 110 kDa NTHi outer membrane protein and the polynucleotide that encodes this outer membrane protein. Methods of screening for NTHi infection, and treating and preventing NTHi related disorders are also contemplated.
1. An isolated polynucleotide comprising the nucleotide sequence of any one of SEQ ID NO: 1.
2. An isolated polynucleotide comprising the nucleotide sequence encoding an amino acid sequence of any one SEQ ID NO: 7.
3. A vector comprising the polynucleotide of claim 1 or 2 .
4. The vector of claim 3 , wherein the polynucleotide is operatively linked to an expression control sequence.
5. A host cell comprising the vector of claim 3 .
6. A host cell comprising the vector of claim 4 .
7. A host cell comprising the polynucleotide of claim 1 or 2 .