CpG single strand deoxynucleotides for use as adjuvant
The present invention provides an adjuvant, which includes at least one single strand deoxynucleotide containing a CpG dinucleotide. The single strand deoxynucleotide comprises one or more CpG dinucleotides. When used in combination with rabies vaccine, HBV vaccine or other vaccines, the adjuvant can significantly improve the immune effect of the vaccine.
1. A method of improving immunogenicity of a vaccine, comprising coadministering said vaccine to a subject with a single strand deoxynucleotide, comprising the nucleotide sequence of SEQ ID NO: 167 and said vaccine.
2. The method of claim 1 , wherein bases of the deoxynucleotide are modified by one or more modifications selected from the group consisting of non-sulfur modification, sulfur modification, partial sulfur modification, rare base modification, methylation modification, and other modifications where sulfhydryl, Aminolinker C6 and Thiol-C6 S-S are used to couple to other substances.
3. The method of claim 1 , wherein the single strand deoxynucleotide is used alone or in combination with a non-nucleic acid adjuvant including aluminum adjuvant, Freund's adjuvant, MPL, or emulsion.
4. The method of claim 1 , wherein the single strand deoxynucleotide is admixed with or chemically coupled to a vaccine, or the single strand deoxynucleotide is cloned into a DNA vaccine.
5. The method of claim 1 , wherein the vaccine is selected from the group consisting of hepatitis B virus blood-derived vaccine, hepatitis B virus genetic engineering protein vaccines, HBV virus vector vaccine, hepatitis B virus bacterium vector vaccine, hepatitis B virus transgenic plant vaccine, rabies virus blood-derived vaccine, rabies virus genetic engineering protein vaccines, rabies virus vector vaccine, rabies virus bacterium vector vaccine, and rabies virus transgenic plant vaccine, and the DNA vaccine is selected from the group consisting of hepatitis B virus DNA vaccine and rabies DNA vaccine.
6. The method of claim 1 , further comprising administering non-nucleic acid adjuvants.
7. The method of claim 1 , wherein the subject is human.
8. The method of claim 1 , wherein the subject is a mammal.