Vascular endothelial cell growth inhibitor, VEGI-192a
This invention discloses two new VEGI isoforms named VEGI- 192a and VEGI- 192b consisting of 192 amino acid residues. These isoforms show endothelial cell-specific expression and share a C-terminal 151-residues segment with the previously described VEGI- 174 and VEGI- 251 . Methods of using these isoforms of VEGI in diagnosing, screening agonist and antagonist of the isoforms, and treating various angiogenesis-related diseases are also disclosed.
1. An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide sequence of SEQ ID NO:4.
2. The polynucleotide of claim 1 , wherein said polynucleotide further comprises a detectable label.
3. The polynucleotide of claim 1 , wherein said polynucleotide is immobilized on a surface.
4. The polynucleotide of claim 1 , wherein said polynucleotide consists of a nucleotide sequence encoding the polypeptide sequence of SEQ ID NO:4.
5. The polynucleotide of claim 1 , wherein said polynucleotide encodes a fusion protein comprising the polypeptide sequence of SEQ ID NO:4 fused to another polypeptide.
6. The polynucleotide of claim 1 , wherein said nucleotide sequence comprises the nucleic acid sequence of SEQ ID NO:1.
7. The polynucleotide of claim 1 , wherein said polynucleotide encodes a polypeptide having a biological activity of anti-angiogenesis.
8. A vector comprising the polynucleotide of claim 1 .
9. The vector of claim 1 , wherein said vector is an expression vector.
10. A host cell in a cell culture, said host cell comprising the vector of claim 8 .
11. A composition comprising the polynucleotide of claim 1 .