Tea polyphenol composition and method for producing the same
The objective of the present invention is to provide a tea polyphenol composition that has reduced bitterness and astringency while containing catechins with high purity. The present invention provides a tea polyphenol composition, (A) comprising 85% by weight to 95% by weight of catechins, (B) wherein the catechins contains epigallocatechin gallate and gallocatechin gallate at a ratio of 65% by weight to 80% by weight and (C) wherein the ratio given by (epigallocatechin gallate+gallocatechin gallate)/(epicatechin+catechin+epigallocatechin+gallocatechin+epicatechin gallate+catechin gallate) is from 2 to 4.
1. A tea polyphenol composition having reduced bitterness and astringency, comprising:
a plurality of at least seven distinct catechins, wherein the catechins are selected from the group consisting of EGCg, GCg, EC, C, EGC, GC, ECg, and Cg;
wherein 85 wt % to 95 wt % of the tea polyphenol composition is comprised of the plurality of catechins;
wherein at least 70 wt % of the tea polyphenol composition is comprised of EC, C, and EGCg together;
wherein EC and C together account for at least 11% of the plurality of distinct catechins, and wherein the tea polyphenol composition is obtained by the process comprising the steps of:
(a) subjecting tea leaves to extraction with hot water at a temperature of from 60° C. to 100° C. to form an aqueous extract;
(b) cooling the aqueous extract to a temperature of 50° C. or lower and concentrating the cooled aqueous extract;
(c) extracting the concentrated aqueous extract with an organic solvent, wherein the organic solvent is acetonitrile, methanol, ethanol, ethyl acetate, acetone, methyl ethyl ketone, methyl isobutly ketone, tetrahydrofuran, dioxane, or a mixture thereof to provide an organic solvent layer;
(d) concentrating the organic solvent layer to provide a concentrated liquid and subsequently drying the concentrated liquid to obtain a crude tea extract;
(e) dissolving the crude tea extract in a 5 to 15% aqueous methanol solution to provide an aqueous methanol extract and adding the aqueous methanol extract to a crosslinked styrenic synthetic adsorption resin, washing the synthetic adsorption resin with 5 to 15% aqueous methanol to wash out unadsorbed components;
(f) eluting and recovering a tea catechin fraction with a 35 to 45% aqueous methanol solution, wherein the recovery of the tea catechin fraction starts at a time when the content of (−)-EGCg has reached a level twice or more as high as that of (−)-EGC and ends at a time when the content of (−)-EGCg has reached a level lower than that of (−)-ECg, or at a time when the content of caffeine becomes 5%; and
(g) concentrating and drying the tea catechin fraction to provide the tea polyphenol composition.
2. The tea polyphenol composition according to claim 1 , wherein (EGCg+GCg) are together present in the polyphenol composition in an amount between 65 wt % to 80 wt %.
3. The tea polyphenol composition according to claim 1 , wherein a wt % ratio of (EGCg+GCg) to (EC, C, EGC, GC, ECg, and Cg) is between 2 and 4.
4. The tea polyphenol composition according to claim 1 , wherein a wt % ratio of (EGCg+ECg+GCg+Cg) to (EC+EGC+GC+C) is between 3 and 7.
5. The tea polyphenol composition according to claim 1 , wherein a wt % ratio of (EGCg+GCg) to (EC+EGC+GC+C) is between 2 and 6.
6. The tea polyphenol composition according to claim 1 , wherein (GCg+GC+Cg+C) are together present in the polyphenol composition in an amount between 2 wt % to 15 wt %.
7. The tea polyphenol composition according to claim 1 , wherein a wt % ratio of EGCg to (EC+EGC+ECg) is between 2 and 4.
8. The tea polyphenol composition according to claim 1 , further comprising caffeine in an amount between 0.0001 wt % and 1.2 wt %.
9. The tea polyphenol composition according to claim 1 , further comprising ash in an amount between 0.0001 wt % and 0.2 wt %.
10. The tea polyphenol composition according to claim 1 , wherein gallic acid is not present in measurable quantities.
11. A tea polyphenol composition, comprising:
a plurality of at least seven distinct catechins, wherein the catechins are selected from the group consisting of EGCg, GCg, EC, C, EGC, GC, ECg, and Cg; wherein
(I) EGCg and GCg are together present in the polyphenol composition in an amount between 65 wt % to 80 wt %;
(II) a wt % ratio of (EGCg+GCg) to (EC, C, EGC, GC, ECg, and Cg) is between 2 and 4;
(III) a wt % ratio of (EGCg+ECg+GCg+Cg) to (EC+EGC+GC+C) is between 3 and 7;
(IV) a wt % ratio of (EGCg+GCg) to (EC+EGC+GC+C) is between 2 and 6;
(V) (GCg+GC+Cg+C) are together present in the polyphenol composition in an amount between 2 wt % to 15 wt %; and
(VI) a wt % ratio of EGCg to (EC+EGC+ECg) is between 2 and 4.
12. The tea polyphenol composition according to claim 11 , further comprising caffeine in an amount between 0.0001 wt % and 1.2 wt %.
13. The tea polyphenol composition according to claim 11 , further comprising ash in an amount between 0.0001 wt % and 0.2 wt %.
14. The tea polyphenol composition according to claim 11 , wherein gallic acid is not present in measurable quantities.