User interface for a fluidic system of a flow cytometer
The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.
1. A fluidic system for pumping sheath fluid and sample fluid into an interrogation zone of a flow cytometer, comprising:
a sheath pump that pumps sheath fluid from a sheath container into an interrogation zone;
a waste pump that pumps waste fluid from the interrogation zone into a waste container;
a drawtube, coupled to the sample container, that conveys the sample fluid from the sample container to the interrogation zone;
wherein the sheath pump and the waste pump cooperate to draw sample fluid from a sample container through the drawtube into the interrogation zone;
a user interface that receives, from a user, an input selected from the group consisting of: desired core stream diameter, core stream fluid type, and estimated sample particle size;
a pressure sensor that measures a pressure differential of the sample fluid between the top of the drawtube and the bottom of the drawtube; and
a controller coupled to the user interface and to the pressure sensor, wherein the controller is configured to adjust the flow rate of the sample fluid from the sample container through the drawtube into the interrogation zone, based on the user input and the measured pressure differential.
2. The fluidic system of claim 1 , further including a storage device coupled to the user interface, wherein the user interface receives and the storage device stores information related to previous sample runs, wherein the stored information includes the type of the core stream fluid, the identification of the sample particle, and flow rate of the sample fluid.
3. The fluidic system of claim 2 , wherein the user interface receives and the storage device stores information related to the identification of the user, the date of the sample run, and exemplary data.
4. The fluidic system of claim 3 , wherein the user interface receives and the storage device stores information related to comments from the user.
5. The fluidic system of claim 1 , further including a core stream detector coupled to the controller that identifies core stream characteristics selected from the group consisting of the actual core stream diameter and time elapsed between the passage of sample particles through the interrogation zone, wherein the controller controls at least one of the flow rates of the sheath fluid and the waste fluid based on the input from the user and the identified core stream characteristics.
6. The fluidic system of claim 1 , wherein the controller adjusts the flow rate of the sample fluid by allowing an adjustable flow rate of the sheath fluid pumped from the sheath container to the interrogation zone.
7. The fluidic system of claim 6 , wherein the controller adjusts the flow rate of the sample fluid by maintaining a consistent flow rate of the waste fluid pumped from the interrogation zone into the waste container.
8. The fluidic system of claim 7 , wherein the controller is a proportional-integral-derivative controller (PID controller), wherein the controller includes a storage device coupled to the user interface, wherein the user interface receives and the storage device stores information related to previous sample runs.
9. The fluidic system of claim 1 , further including a core stream detector coupled to the controller that identifies core stream characteristics, wherein the controller controls at least one of the flow rates of the sheath fluid and the waste fluid further based on the input from the user and the identified core stream characteristics.
10. The fluidic system of claim 9 , wherein the core stream characteristics are selected from the group consisting of the actual core stream diameter, time elapsed between the passage of sample particles through the interrogation zone, and flow rate of sample particles through the interrogation zone.
11. The fluidic system of claim 1 , further comprising a storage device coupled to the user interface, wherein the user interface receives and the storage device stores information related to previous sample runs, wherein the stored information includes the type of the core stream fluid, the identification of the sample particle, and flow rate of the sample fluid.
12. The fluidic system of claim 11 , wherein the user interface receives and the storage device stores information related to the identification of the user, the date of the sample run, exemplary data, and comments from the user.