Detection of post-translationally modified peptides with liquid crystals
A method for differentiating between a post-translationally modified peptide and a peptide contained in a sample, comprising: (a) contacting the sample with a peptide attachment surface to create a peptidized surface, wherein the sample includes at least one functional group; (b) contacting the peptidized surface with a recognition reagent that selectively binds or forms a complex with the post-translationally modified peptide in the sample to provide an incubated surface; and (c) contacting a liquid crystal with the incubated surface and detecting presence of post-translationally modified peptide in the sample with the liquid crystal.
1. A device for differentiating between a post-translationally modified peptide and a peptide, comprising:
(a) a support having a top surface;
(b) metal overlying the top surface of the support providing a metallized surface;
(c) a functionalized thiol compound comprising the reaction product of a first thiol compound comprising a —S-Metal linkage formed when a thiol compound comprising an —SH group is attached to the top of the metallized surface and further comprising an amine or ammonium group or an ammonium salt thereof linked to a heterobifunctional linker comprising a functional group that is capable of reacting with an —SH group on a post-translationally modified peptide or a peptide; and
(d) a liquid crystal in contact with the functionalized thiol compound of (c) above,
wherein the post-translationally modified peptide, and the peptide are bound to the functionalized thiol compound of (c) and wherein the orientation of the liquid crystal in contact with the post-translationally modified peptide is different than the orientation of the liquid crystal in contact with the peptide, thereby differentiating between the post-translationally modified peptide and the peptide.
2. The device of claim 1 , further comprising:
(e) a second thiol compound comprising an —S-Metal linkage formed when a thiol compound comprising an —SH group is attached to the top of the metallized surface, wherein the second thiol compound is a compound having the formula Metal-S—(CH 2 ) c —(OCH 2 CH 2 ) d —X, wherein c is an integer ranging from 1 to 30, and d is an integer ranging from 0 to 10, and X is selected from an —OH, an alkoxy group, a CH 3 , a sugar, a zwitterionic group, or a polar non-ionic group.
3. The device of claim 1 , wherein the metallized surface comprises a top layer of gold and wherein the top layer of gold overlies a layer of titanium that promotes adhesion of the gold to the support.
4. A kit, comprising the device of claim 1 and a recognition reagent, wherein the recognition reagent is an antibody, and antibody fragment, or a cationic compound.
5. A kit, comprising the device of claim 1 , and a recognition reagent, wherein the recognition reagent is an antibody, an antibody fragment, a cationic surfactant, a polyelectrolyte, a cationic iron compound, or a phosphosensor dye.
6. The device of claim 1 , wherein the recognition reagent is an antibody or an antibody fragment that selectively binds or forms a complex with a post-translationally modified peptide.
7. The device of claim 2 wherein the molar ratio of the functionalized thiol compound of (c) to the second thiol compound attached to the top surface of the support ranges from 0.1:99.9 to 50:50.
8. The device of claim 1 wherein the functionalized thiol compound of (c) has the formula:
wherein e is an integer ranging from 1 to 30 and f is an integer ranging from 0 to 10.