Analytical method and kit thereof
The present invention relates to a peptide comprising a symmetrical dimethylated arginine, and constitute an immunologic determinant of antibodies present in sera from patients with systemic lupus erythematosus (SLE), and wherein the methylation is a prerequisite for reacting with said antibodies. The invention also relates to the use of said peptide for diagnosis of SLE and the differentiation between SLE and MCTD.
1. A peptide consisting of the amino acid sequence
AARGsdRGRGMGRGNIF (SEQ ID NO: 1)
wherein the amino acid sdR is symmetric dimethylated arginine having the structure
and wherein the peptide is able to react with antibodies which are presented in sera from patients with systemic lupus erythematosus (SLE).
2. A method of diagnosing systemic lupus erythematosus (SLE), comprising contacting sera of a patient with a composition comprising the peptide of claim 1 .
3. The method according to claim 2 , wherein the diagnosis is differential diagnosis to distinguish between SLE patients and patients with mixed connective tissue disease (MCTD).
4. The method according to claim 2 , wherein the diagnosis is an in vitro diagnosis of SLE.
5. The method according to claim 2 , wherein said composition is used for differentiation between SLE and mixed connective tissue disease (MCTD).
6. A peptide comprising multimers of the peptide of claim 1 .
7. A kit for detection of antibodies, comprising the peptide of Claim 1 .
8. A kit according to claim 7 , wherein said peptide is used for in vitro diagnosis of SLE.
9. A kit according to claim 7 , wherein said peptide is used for differential diagnosis to distinguish between SLE and mixed connective tissue disease (MCTD).
10. A method for monitoring a disease activity comprising repeated testing to follow the titer of antibodies able to react with the peptide according to claim 1 in order to monitor the effect of treatment or the disease activity, wherein the disease is SLE.
11. The method according to claim 10 , wherein said disease is dsDNA negative SLE.