Holographic microfabrication and characterization system for soft matter and biological systems
A method and system for micromanipulation of objects of any shape. The method and system creates various forms of holographic optical traps for a variety of commercial purposes. Some alternate forms of traps include a dark form of optical traps, optical vortices with different helical winding numbers and optical traps with variable phase profiles imprinted thereon.
1. An optical imaging system for examining an object comprising:
a light source to produce a light beam;
a spatial light modulator;
an optical train for processing the light beam to produce a plurality of optical traps;
the optical train including an objective lens and a relay lens with a transfer function provided by the spatial light modulator generating a phase only hologram thereby providing an imaging train focused to a plane at height z above an objective lens' focal plane by imprinting a same hologram phase pattern used to form the optical traps, thereby enabling an imaging plane to be effectively scanned through the object in three dimensions without moving the object relative to the objective lens.
2. The optical system as defined in claim 1 wherein the phase only hologram operates to correct geometric aberrations in the optical train.
3. The optical system as defined in claim 1 further including a video camera which is synchronized to the spatial light modulator to gather volumetric data about the object at video rates.
4. The optical system as defined in claim 1 further including a beam block disposed into an intermediate focal plane of the relay lens thereby converting the optical train to dark field operation.
5. The optical system as defined in claim 1 further including a white light source for the light source, a dielectric multi-layer mirror and a second objective lens positioned operatively as a condenser, thereby enabling white light imaging of the object.
6. The optical system as defined in claim 1 further including a fiber spectrometer and the light source comprising two white light sources to provide transmission and reflection spectra in real time, thereby enabling fine tuning of structures associated with the object prior to fixing the structures.
7. The optical system as defined in claim 2 further including an optical subsystem disposed below the imaging system to provide simultaneous trapping by the optical traps, imaging and real-time spectroscopy.