The invention discloses the Chlamydia PMPE and PMPI polypeptide, polypeptides derived therefrp, (PMP-derived polypeptides), nucleotide sequences encoding said polypeptides, antibodies that specifically bind the PMP polypeptides and PMP-derived polypeptides and T-cells specific for PMP polypeptides and PMP-derived polypeptides. Also disclosed are prophylactic and therapeutic compositions, including immunogenic compositions, e.g., vaccines, comprising PMP polypeptides or PMP-derived polypeptides or antibodies thereto. The invention additionally discloses methods of inducing in animals an immune response to Chlamydia cells, Chlamydia elementary bodies, and/or cells expressing Chlamydial proteins, e.g., cell infected with Chlamydia.
1. An isolated polynucleotide comprising the nucleic acid sequence which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of:
(a) amino acids 32-965 of SEQ ID NO: 2;
(b) SEQ ID NO: 2;
(c) amino acids 590-625 of SEQ ID NO: 2;
(d) amino acids 1-500 of SEQ ID NO: 2;
(e) amino acids 625-700 of SEQ ID NO: 2;
(f) the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462; and
(g) a combination of two or more amino acid sequences of (a)-(f).
2. The polynucleotide of claim 1 , wherein said polypeptide further comprises a heterologous polypeptide.
3. The polynucleotide of claim 2 , wherein said heterologous polypeptide is selected from the group consisting of a pre- and/or pro-sequence, an affinity purification sequence, and an immunogenic sequence.
4. The polynucleotide of claim 2 , wherein said heterologous polypeptide is selected from the group consisting of SEQ ID NO: 4; amino acids 13-40 of SEQ ID NO: 4; amino acids 70-110 of SEQ ID NO: 4; amino acids 150-225 of SEQ ID NO: 4; amino acids 250-290 of SEQ ID NO: 4; amino acids 370-455 of SEQ ID NO: 4; amino acids 400-455 of SEQ ID NO: 4; amino acids 470-520 of SEQ ID NO: 4; amino acids 615-670 of SEQ ID NO: 4; amino acids 710-775 of SEQ ID NO: 4; amino acids 765-825 of SEQ ID NO: 4; amino acids 830-860 of SEQ ID NO: 4; amino acids 1-500 of SEQ ID NO: 4; and the amino acid sequence encoded by the Chlamydia insert in E. coli TOP10 assigned as ATCC PTA No. 2461.
5. The polynucleotide of claim 1 , wherein said nucleic acid sequence is selected from the group consisting of:
(a) nucleotides 94-2895 of SEQ ID NO: 1;
(b) SEQ ID NO: 1;
(c) the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462; and
(d) a combination of (a), (b), or (c), or the complement thereof.
6. A vector comprising the polynucleotide of claim 1 .
7. A host cell comprising the vector of claim 6 .
8. A composition comprising the polynucleotide of claim 1 and a carrier.
9. The composition of claim 8 , further comprising an adjuvant.
10. An isolated polynucleotide comprising the nucleic acid sequence, which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 4 or the amino acid sequence encoded by the Chlamydia insert in E. coli TOP10 assigned as ATCC PTA No. 2461.
11. The polynucleotide of claim 10 , wherein said polypeptide further comprises a heterologous polypeptide.
12. The polynucleotide of claim 11 , wherein said heterologous polypeptide is selected from the group consisting of a pre- and/or pro-sequence, an affinity purification sequence, and an immunogenic sequence.
13. The polynucleotide of claim 11 , wherein said heterologous polypeptide is selected from the group consisting of SEQ ID NO: 2; amino acids 32-965 of SEQ ID NO: 2; amino acids 15-56 of SEQ ID NO: 2; amino acids 15-121 of SEQ ID NO: 2; amino acids 45-125 of SEQ ID NO: 2; amino acids 125-190 of SEQ ID NO: 2; amino acids 195-261 of SEQ ID NO: 2; amino acids 275-366 of SEQ ID NO: 2; amino acids 375-440 of SEQ ID NO: 2; amino acids 440-490 of SEQ ID NO: 2; amino acids 525-590 of SEQ ID NO: 2; amino acids 590-625 of SEQ ID NO: 2; amino acids 615-650 of SEQ ID NO: 2; amino acids 625-700 of SEQ ID NO: 2; amino acids 725-800 of SEQ ID NO: 2; amino acids 755-775 of SEQ ID NO: 2; amino acids 785-845 of SEQ ID NO: 2; amino acids 815-865 of SEQ ID NO: 2; amino acids 1-31 of SEQ ID NO: 2; amino acids 1-500 of SEQ ID NO: 2; and the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462.
14. The polynucleotide of claim 10 , wherein said nucleic acid sequence is SEQ ID NO:3 or the Chlamydia insert in E. coli TOP10 assigned as ATCC PTA No. 2461.
15. A vector comprising the polynucleotide of claim 10 .
16. A host cell comprising the vector of claim 15 .
17. A composition comprising the polynucleotide of claim 10 and a carrier.
18. The composition of claim 17 , further comprising an adjuvant.
19. An isolated polynucleotide encoding a polypeptide, which consists of the amino acid sequence selected from the group consisting of: amino acids 32-965 of SEQ ID NO: 2; SEQ ID NO: 2; the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462; amino acids 15-56 of SEQ ID NO: 2; amino acids 15-121 of SEQ ID NO: 2; amino acids 45-125 of SEQ ID NO: 2; amino acids 125-190 of SEQ ID NO: 2; amino acids 195-261 of SEQ ID NO: 2; amino acids 275-366 of SEQ ID NO: 2; amino acids 375-440 of SEQ ID NO: 2; amino acids 440-490 of SEQ ID NO: 2; amino acids 525-590 of SEQ ID NO: 2; amino acids 590-625 of SEQ ID NO: 2; amino acids 615-650 of SEQ ID NO: 2; amino acids 625-700 of SEQ ID NO: 2; amino acids 725-800 of SEQ ID NO: 2; amino acids 755-775 of SEQ ID NO: 2; amino acids 785-845 of SEQ ID NO: 2; amino acids 815-865 of SEQ ID NO: 2; amino acids 1-31 of SEQ ID NO: 2; and amino acids 1-500 of SEQ ID NO: 2.
20. A vector comprising the polynucleotide of claim 19 .
21. A host cell comprising the vector of claim 20 .
22. A composition comprising the polynucleotide of claim 19 and a cattier.
23. The composition of claim 22 , further comprising an adjuvant.
24. The composition of claim 22 , further comprising a heterologous polynucleotide.
25. The composition of claim 24 , wherein said heterologous polynucleotide encodes the amino acid sequence, which is selected from the group consisting of SEQ ID NO: 4; amino acids 13-40 of SEQ ID NO: 4; amino acids 70-110 of SEQ ID NO: 4; amino acids 150-225 of SEQ ID NO: 4; amino acids 250-290 of SEQ ID NO: 4; amino acids 370-455 of SEQ ID NO: 4; amino acids 400-455 of SEQ ID NO: 4; amino acids 470-520 of SEQ ID NO: 4; amino acids 615-670 of SEQ ID NO: 4; amino acids 710-775 of SEQ ID NO: 4; amino acids 765-825 of SEQ ID NO: 4; amino acids 830-860 of SEQ ID NO: 4; amino acids 1-500 of SEQ ID NO: 4; and the amino acid sequence encoded by the Chlamydia insert in E. coli TOP10 assigned as ATCC PTA No. 2461.
26. An isolated polynucleotide encoding a polypeptide, which consists of the amino acid sequence selected from the group consisting of: SEQ ID NO: 4; amino acids 13-40 of SEQ ID NO: 4; amino acids 70-110 of SEQ ID NO: 4; amino acids 150-225 of SEQ ID NO: 4; amino acids 250-290 of SEQ ID NO: 4; amino acids 370-455 of SEQ ID NO: 4; amino acids 400-455 of SEQ ID NO: 4; amino acids 470-520 of SEQ ID NO: 4; amino acids 615-670 of SEQ ID NO: 4; amino acids 710-775 of SEQ ID NO: 4; amino acids 765-825 of SEQ ID NO: 4; amino acids 830-860 of SEQ ID NO: 4; and amino acids 1-500 of SEQ ID NO: 4.
27. A vector comprising the polynucleotide of claim 26 .
28. A host cell comprising the vector of claim 27 .
29. A composition comprising the polynucleotide of claim 26 and a carrier.
30. The composition of claim 29 , further comprising an adjuvant.
31. The composition of claim 29 , further comprising a heterologous polynucleotide.
32. The composition of claim 31 , wherein said heterologous polynucleotide encodes the amino acid sequence, which is selected from the group consisting of SEQ ID NO: 2; amino acids 15-56 of SEQ ID NO: 2; amino acids 15-121 of SEQ ID NO: 2; amino acids 45-125 of SEQ ID NO: 2; amino acids 125-190 of SEQ ID NO: 2; amino acids 195-261 of SEQ ID NO: 2; amino acids 275-366 of SEQ ID NO: 2; amino acids 375-440 of SEQ ID NO: 2; amino acids 440-490 of SEQ ID NO: 2; amino acids 525-590 of SEQ ID NO: 2; amino acids 590-625 of SEQ ID NO: 2; amino acids 615-650 of SEQ ID NO: 2; amino acids 625-700 of SEQ ID NO: 2; amino acids 725-800 of SEQ ID NO: 2; amino acids 755-775 of SEQ ID NO: 2; amino acids 785-845 of SEQ ID NO: 2; amino acids 815-865 of SEQ ID NO: 2; amino acids 1-31 of SEQ ID NO: 2; amino acids 1-500 of SEQ ID NO: 2; and the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462.
33. An isolated polynucleotide comprising a nucleotide sequence, which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of: amino acids 32-965 of SEQ ID NO: 2; SEQ ID NO: 2; the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462; amino acids 15-56 of SEQ ID NO: 2; amino acids 15-121 of SEQ ID NO: 2; amino acids 45-125 of SEQ ID NO: 2; amino acids 125-190 of SEQ ID NO: 2; amino acids 195-261 of SEQ ID NO: 2; amino acids 275-366 of SEQ ID NO: 2; amino acids 375-440 of SEQ ID NO: 2; amino acids 440-490 of SEQ ID NO: 2; amino acids 525-590 of SEQ ID NO: 2; amino acids 590-625 of SEQ ID NO: 2; amino acids 615-650 of SEQ ID NO: 2; amino acids 625-700 of SEQ ID NO: 2; amino acids 725-800 of SEQ ID NO: 2; amino acids 755-775 of SEQ ID NO: 2; amino acids 785-845 of SEQ ID NO: 2; amino acids 815-865 of SEQ ID NO: 2; amino acids 1-31 of SEQ ID NO: 2; and amino acids 1-500 of SEQ ID NO: 2, wherein said polypeptide further comprises a heterologous polypeptide.
34. The polynucleotide of claim 33 , wherein said heterologous polypeptide is selected from the group consisting of a pre- and/or pro-sequence, an affinity purification sequence, and an immunogenic sequence.
35. The polynucleotide of claim 33 , wherein said heterologous polypeptide comprises the amino acid sequence selected from the group consisting of SEQ ID NO: 4; amino acids 13-40 of SEQ ID NO: 4; amino acids 70-110 of SEQ ID NO: 4; amino acids 150-225 of SEQ ID NO: 4; amino acids 250-290 of SEQ ID NO: 4; amino acids 370-455 of SEQ ID NO: 4; amino acids 400-455 of SEQ ID NO: 4; amino acids 470-520 of SEQ ID NO: 4; amino acids 615-670 of SEQ ID NO: 4; amino acids 710-775 of SEQ ID NO: 4; amino acids 765-825 of SEQ ID NO: 4; amino acids 830-860 of SEQ ID NO: 4; amino acids 1-500 of SEQ ID NO: 4; and the amino acid sequence encoded by the Chlamydia insert in E. coli TOP10 assigned as ATCC PTA No. 2461.
36. A vector comprising the polynucleotide of claim 33 .
37. A host cell comprising the vector of claim 36 .
38. A composition comprising the polynucleotide of claim 33 and a carrier.
39. An isolated polynucleotide comprising a nucleotide sequence, which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of: amino acids 13-40 of SEQ ID NO: 4; amino acids 70-110 of SEQ ID NO: 4; amino acids 150-225 of SEQ ID NO: 4; amino acids 250-290 of SEQ ID NO: 4; amino acids 370-455 of SEQ ID NO: 4; amino acids 400-455 of SEQ ID NO: 4; amino acids 470-520 of SEQ ID NO: 4; amino acids 615-670 of SEQ ID NO: 4; amino acids 710-775 of SEQ ID NO: 4; amino acids 765-825 of SEQ ID NO: 4; amino acids 830-860 of SEQ ID NO: 4; and amino acids 1-500 of SEQ ID NO: 4, wherein said polypeptide further comprises a heterologous polypeptide.
40. The polynucleotide of claim 39 , wherein said heterologous polypeptide is selected from the group consisting of a pre- and/or pro-sequence, an affinity purification sequence, and an immunogenic sequence.
41. The polynucleotide of claim 39 , wherein said heterologous polypeptide comprises the amino acid sequence selected from the group consisting of SEQ ID NO: 2; amino acids 15-56 of SEQ ID NO: 2; amino acids 15-121 of SEQ ID NO: 2; amino acids 45-125 of SEQ ID NO: 2; amino acids 125-190 of SEQ ID NO: 2; amino acids 195-261 of SEQ ID NO: 2; amino acids 275-366 of SEQ ID NO: 2; amino acids 375-440 of SEQ ID NO: 2; amino acids 440-490 of SEQ ID NO: 2; amino acids 525-590 of SEQ ID NO: 2; amino acids 590-625 of SEQ ID NO: 2; amino acids 615-650 of SEQ ID NO: 2; amino acids 625-700 of SEQ ID NO: 2; amino acids 725-800 of SEQ ID NO: 2; amino acids 755-775 of SEQ ID NO: 2; amino acids 785-845 of SEQ ID NO: 2; amino acids 815-865 of SEQ ID NO: 2; amino acids 1-31 of SEQ ID NO: 2; amino acids 1-500 of SEQ ID NO: 2; and the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462.
42. A vector comprising the polynucleotide of claim 39 .
43. A host cell comprising the vector of claim 42 .
44. A composition comprising the polynucleotide of claim 39 and a carrier.