Methods of identifying a subject having or at risk of having or developing coronary artery disease
View Patent ↗Provided herein are compositions and methods for identifying individuals at risk for developing coronary artery disease (CAD).
1. A method for determining a subject's predisposition to a coronary artery disease, the method comprising:
a) determining the subject's plasma OxPL level, wherein the OxPL is associated with apolipoprotein B-100 (apoB) particles;
b) determining the subject's Lp-PLA 2 mass or activity; and
c) correlating a) and b), wherein increased levels of OxPL and Lp-PLA 2 are indicative of a predisposition to the coronary artery disease.
2. The method of claim 1 , further comprising determining the presence of a pro-inflammatory genotype in the IL-1 gene cluster of the subject.
3. The method of claim 2 , wherein determining the IL-1 gene cluster genotype of the subject comprises identifying a pro-inflammatory single nucleotide polymorphism in the IL-1A, IL-1B, or IL-1B gene, or any combination thereof.
4. The method of claim 3 , wherein the single nucleotide polymorphism in IL-1A is associated with the nucleotide at position +4845.
5. The method of claim 4 , wherein the nucleotide at position +4845 is not G.
6. The method of claim 4 , wherein the nucleotide at position +4845 is T.
7. The method of claim 3 , wherein the single nucleotide polymorphism in IL-1B is associated with the nucleotide at position +3954.
8. The method of claim 7 , wherein the nucleotide at position +3954 is not C.
9. The method of claim 7 , wherein the nucleotide at position +3954 is T.
10. The method of claim 3 , wherein the single nucleotide polymorphism in IL-1B is associated with the nucleotide at position −511.
11. The method of claim 10 , wherein the nucleotide at position −511 is not C.
12. The method of claim 10 , wherein the nucleotide at position −511 is T.
13. The method of claim 1 , wherein the determination is independent of C-reactive protein activity.
14. The method of claim 1 , wherein the subject is a human subject.
15. A method of identifying a subject having or at risk of having or developing a coronary artery disease , comprising
(1)measuring in a sample from the subject at least:
(a) Lp-PLA 2 or sPLA 2 activity or mass in the sample; and
(b) oxidized phospholipids (OxPL) on apolipoprotein B-100 particles (apoB), and
(2) correlating the measurements of (1) (a) and (1) (b) with a risk of having or developing a coronary artery disease.
16. A method of identifying a subject having or at risk of having or developing a coronary artery disease, comprising
(1) measuring in a sample from the subject at least:
(a) Lp-PLA 2 activity or mass in the sample,
wherein the Lp-PLA 2 activity is measured in a fluorimetric assay comprising a substrate for Lp-PLA 2 ; and
(b) oxidized phospholipids (OxPL) on apolipoprotein B-100 particles (apoB), and
(2) correlating the measurements of (1)(a) and (1)(b) with a risk of having or developing a coronary artery disease.