Dominant B cell epitopes and methods of making and using thereof
View Patent ↗Disclosed are methods for obtaining at least one epitope suitable for detecting the presence of an antibody against a tumor associated antigen of a cancer in a sample. Kits, assays, and substrates employing the epitopes of the present invention are disclosed. Also disclosed are epitopes of NY-ESO-1 and XAGE-1b and methods of using thereof.
1. An isolated peptide consisting of SEQ ID NO:16.
2. A composition comprising the isolated peptide of claim 1 .
3. A substrate which comprises the isolated peptide of claim 1 immobilized thereon.
4. A kit comprising the isolated peptide of claim 1 packaged together with at least one reagent.
5. An isolated complex comprising the isolated peptide of claim 1 and an antibody which selectively binds the peptide.
6. A method for assaying, enriching, isolating or purifying at least one antibody against a tumor associated antigen, or a B cell producing the antibody, which comprises contacting the antibody with the isolated peptide of claim 1 .
7. A method which comprises contacting the isolated peptide of claim 1 with a sample and detecting whether an antibody in the sample selectively binds the peptide.
8. A method for detecting, diagnosing, or monitoring lung cancer in a subject which comprises contacting the isolated peptide of claim 1 with a sample obtained from the subject and detecting whether an antibody in the sample selectively binds the peptide.
9. The method of claim 8 , wherein a plurality of samples otained from the subject are tested.
10. The method of claim 9 , wherein the plurality of samples are obtained before, during or after treating the lung cancer.
11. The method of claim 10 , wherein, the regression, recurrence or progression of the lung cancer is determined by observing any change or difference in the antibody selectively binding the peptide from the plurality of samples.