Miniaturized microtiter plate for HT-screening
The invention relates to a miniaturized microtiter plate which has a body made of plastic and a base made of glass, 1000 to 4000 vessels (wells) ( 3 ), and a cover ( 4 ) which prevents evaporation. The diameter of the vessels (wells) (d) is approximately 1.0 to 1.8 mm. The base of the microtiter plate ( 2 ) has a layer thickness ranging from 0.07 to 0.2 mm and the distance of the outermost vessels (wells) from the edge of the glass base ranges from 4 to 11 mm.
1. A miniaturized microtiter plate with vessels said plate having:
a frame with a length of 127 mm, a width of 85 mm and a height c about 6-20 mm;
a lid with projections;
a glass base fastened to an underside of said frame;
an edge distance a from said lid outer edge width to said projection about 3-8 mm;
an edge distance b from said lid outer edge width to a periphery of a vessel proximal to said outer edge about 6-11 mm;
an edge distance a′ from said lid outer edge length to said projection about 3-8 mm;
an edge distance b′ from said lid outer edge length to a periphery of a vessel proximal to said outer edge about 4-11 mm;
a distance between the center of a vessel proximal to said outer edge and an edge of said glass base about 4-11 mm;
a frame height c1 from the bottom of the frame to the glass base about 3-12 mm;
said vessels having a diameter between about 1.0 and 1.8 mm;
a distance between vessel centers about 2.25 mm; and
a vessel height between 2.0 and 7.0 mm,
whereby the microtiter plate has a body made of said frame, said base and said lid.
2. The miniaturized microtiter plate of claim 1 wherein the edge distance a is about 6 mm.
3. The miniaturized microtiter plate of claim 1 wherein the edge distance b is about 9.5 mm.
4. The miniaturized microtiter plate of claim 1 wherein the edge distance a′ is about 6 mm.
5. The miniaturized microtiter plate of claim 1 wherein the edge distance b′ is about 6.5 mm.
6. The miniaturized microtiter plate of claim 1 wherein said height is about 6-15 mm.
7. The miniaturized microtiter plate of claim 6 wherein said height is about 6 mm.
8. The miniaturized microtiter plate of claim 1 wherein said frame height from the bottom of the frame to the glass base about 3 mm.
9. The miniaturized microtiter plate of claim 1 wherein said diameter is about 1.3 mm.
10. A method for high throughput screening using confocal optics comprising:
providing a sample in a miniaturized microtiter plate with vessels said plate having:
a frame with a length of 127 mm, a width of 85 mm and a height c about 6-20 mm;
a lid with projections;
a glass base fastened to an underside of said frame;
an edge distance a from said lid outer edge width to said projection about 3-8 mm;
an edge distance b from said lid outer edge width to a periphery of a vessel proximal to said outer edge about 6-11 mm;
an edge distance a′ from said lid outer edge length to said projection about 3-8 mm;
an edge distance b′ from said lid outer edge length to a periphery of a vessel proximal to said outer edge about 4-11 mm;
a distance between the center of a vessel proximal to said outer edge and an edge of said glass base about 4-11 mm;
a frame height c1 from the bottom of the frame to the glass base about 3-12 mm;
said vessels having a diameter between about 1.0 and 1.8 mm;
a distance between vessel centers about 2.25 mm; and
a vessel height between 2.0 and 7.0 mm,
whereby the microtiter plate has a body made of said frame, said base and said lid positioning said plate for analysis by a confocal optics device; and analyzing said sample using the confocal optics device.