Diluent for norovirus or sapovirus specimen and method for detecting virus
The present invention relates to a diluent for a Norovirus or Sapovirus specimen, the diluent containing an alkaline buffer of pH 9.0 to 10.0, and to a method for detecting a Norovirus or a Sapovirus through use of the diluent. According to the present invention, a Norovirus or a Sapovirus can be detected in a Norovirus- or Sapovirus-containing specimen such as stool, vomit, body fluid, blood, body tissue, or food, in a convenient manner without use of a special machine such as a centrifuge, with an improved detection sensitivity, and with complete elimination of non-specific factors.
1. A method for detecting a Norovirus in a specimen comprising:
providing a specimen in an alkaline specimen buffer at a pH ranging from 9 to 10;
contacting said specimen with an immobilized anti-Norovirus antibody for a time and under conditions sufficient for binding to occur, wherein contacting between the specimen and antibody takes place at a pH ranging from 9 to 10; and
detecting binding between the specimen and the anti-Norovirus antibody thereby detecting Norovirus in the specimen;
wherein said alkaline specimen buffer optionally comprises at least one ingredient selected from the group consisting of an animal globulin, a surfactant, a water-soluble polymer and a salt.
2. The method of claim 1 , further comprising contacting said specimen with a second labeled anti-Norovirus antibody.
3. The method of claim 1 , which is a sandwich method wherein said specimen is contacted with an immobilized anti-Norovirus antibody and then simultaneously or subsequently contacted with labeled anti-Norovirus antibody.
4. The method of claim 1 , wherein said specimen is a food.
5. The method of claim 1 , wherein said specimen is a bodily tissue, blood or another bodily fluid, vomit or stool.
6. The method of claim 1 , wherein said specimen buffer contains an animal globulin.
7. The method of claim 1 , wherein said specimen buffer contains a surfactant.
8. The method of claim 1 , wherein said specimen buffer contains a water-soluble polymer.
9. The method of claim 1 , wherein said specimen buffer contains a salt.
10. A method for detecting a Sapovirus in a specimen comprising:
providing a specimen in an alkaline specimen buffer at a pH ranging from 9 to 10;
contacting said specimen with an immobilized anti-Sapovirus antibody for a time and under conditions sufficient for binding to occur, wherein contacting between the specimen and antibody takes place at a pH ranging from 9 to 10; and
detecting binding between the specimen and the anti-Sapovirus antibody thereby detecting Sapovirus in the specimen;
wherein said alkaline specimen buffer optionally comprises at least one ingredient selected from the group consisting of an animal globulin, a surfactant, a water-soluble polymer and a salt.
11. The method of claim 10 , further comprising contacting said specimen with a second labeled anti-Sapovirus antibody.
12. The method of claim 10 , which is a sandwich method wherein said specimen is contacted with an immobilized anti-Sapovirus antibody and then simultaneously or subsequently contacted with labeled anti-Sapovirus antibody.
13. The method of claim 10 , wherein said specimen is a food.
14. The method of claim 10 , wherein said specimen is a bodily tissue, blood or another bodily fluid, vomit or stool.
15. The method of claim 10 , wherein said specimen buffer contains an animal globulin.
16. The method of claim 10 , wherein said specimen buffer contains a surfactant.
17. The method of claim 10 , wherein said specimen buffer contains a water-soluble polymer.
18. The method of claim 10 , wherein said specimen buffer contains a salt.