Two-photon fluorescent probes for acidic vesicles in live cells and tissue and method of imaging acidic vesicles in live cells and tissue using the same
View Patent ↗Provided are two-photon fluorescent probes for imaging acidic vesicles in live cells and tissue. The probes are represented by The probes can selectively bind to vesicles in cytosol to emit two-photon excited fluorescence with high intensity. Therefore, the use of the probes enables effective imaging of acidic vesicles. Further provided is a method for imaging acidic vesicles in live cells and tissue using the probes.
1. A two-photon fluorescent probe for imaging acidic vesicles in live cells and tissue, represented by Formula 1:
wherein R 1 is (CH 3 ) 2 NCH 2 CH 2 — or
(R 2 is a hydrogen atom or a methoxy group).
2. The two-photon fluorescent probe according to claim 1 , wherein the probe is excited by light with a wavelength of 780 nm and has a two-photon action cross section (Φδ) of at least 86 GM.
3. The two-photon fluorescent probe according to claim 1 , wherein the probe shows two-photon emission spectra whose fluorescence intensity increases with decreasing pH of cells to be visualized.
4. The two-photon fluorescent probe according to claim 1 , wherein the probe has a pK a of 4 to 5.
5. The two-photon fluorescent probe according to claim 1 , wherein the probe has a water solubility of at least 5.0 μM.
6. The two-photon fluorescent probe according to claim 1 , wherein the absorption spectra of the probe show bathochromic shifts with increasing solvent polarity.
7. The two-photon fluorescent probe according to claim 1 , wherein the probe visualizes acidic vesicles at a penetration depth of 250 μm.
8. A method for imaging acidic vesicles in live cells and tissue, the method comprising introducing the probe according to claim 1 into cytosol to be visualized and observing two-photon excited fluorescence images emitted from the probe.