Permethylation of oligosaccharides
View Patent ↗A solid-phase permethylation procedure is described. For example, solid-phase permethylation can be utilized to prepare permethylated linear and branched, neutral and sialylated oligosaccharides, which can be analyzed by MALDI-MS.
1. A method for conducting solid-phase permethylation of oligosaccharides in a reactor, the method comprising:
a) infusing a polar, aprotic solvent through a packed inorganic base in the reactor, wherein the solvent includes an oligosaccharide and a source of methyl groups;
b) contacting the oligosaccharide with the source of methyl groups; and
c) collecting a permethylated oligosaccharide.
2. The method of claim 1 , wherein the reactor comprises:
a) a container;
b) the base disposed within the container; and
c) the solvent disposed within the container.
3. The method of claim 2 , wherein the infusing solvent is diposed within the container during permethylation.
4. The method of claim 2 , wherein the infusing solvent is delivered to the container during permethylation.
5. The method of claim 2 , wherein the container is a fused-silica capillary.
6. The method of claim 2 , wherein the base is NaOH.
7. The method of claim 2 , wherein the solvent is DMSO.
8. The method of claim 2 , wherein the source of methyl groups is methyl iodide.
9. The method of claim 2 , wherein infusing is carried out by centrifugation.
10. The method of claim 2 , wherein infusing is carried out by a means including a syringe.
11. The method of claim 1 , wherein the reactor is a microreactor.
12. The method of claim 2 , wherein the reactor is a microreactor.