Identification of
View Patent ↗Antibodies, polypeptides, and polynucleotides are provided for the detection, prevention, amelioration and treatment of diseases caused by Actinobacillus actinomycetemcomitans.
1. A method of detecting the presence of Actinobacillus actinomycetemcomitans or an A. actinomycetemcomitans antigen in a test sample comprising contacting the test sample with an antibody or a fragment thereof, wherein the fragment thereof is a Fab, Fab′, Fab′-SH, F(ab′) 2 , or Fv fragment, wherein the antibody or fragment thereof specifically binds to a polypeptide consisting of SEQ ID NO:22, and detecting an immunocomplex comprising A. actinomycetemcomitans or an A. actinomycetemcomitans antigen and the antibody or fragment thereof, wherein detection of the immunocomplex indicates the presence of A. actinomycetemcomitans or an A. actinomycetemcomitans antigen in the test sample.
2. The method of claim 1 , wherein the test sample is plaque, saliva, crevicular fluid, sputum, blood, serum, plasma, urine, feces, cerebrospinal fluid, amniotic fluid, wound exudate, or tissue.
3. The method of claim 1 , wherein the antibody or fragment thereof is bound to a solid substrate.
4. The method of claim 1 , wherein the method is an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a western blot assay.
5. A method for detecting an antibody specific for A. actinomycetemcomitans in a test sample comprising contacting the test sample with a purified polypeptide comprising SEQ ID NO:22 and detecting formation of an immunocomplex comprising the polypeptide of SEQ ID NO:22 and the antibody specific for A. actinomycetemcomitans , wherein detection of the immunocomplex indicates the presence of an antibody specific for A. actinomycetemcomitans in the test sample.
6. The method of claim 5 , wherein the test sample is contacted with a purified polypeptide consisting of SEQ ID NO:22.
7. The method of claim 5 , wherein the test sample is plaque, saliva, crevicular fluid, sputum, blood, serum, plasma, urine, feces, cerebrospinal fluid, amniotic fluid, wound exudate, or tissue.
8. The method of claim 5 , wherein the polypeptide is bound to a solid substrate.
9. The method of to claim 5 , wherein the method is an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a western blot assay.
10. A method for detecting an antibody specific for A. actinomycetemcomitans in a test sample comprising contacting the test sample with a purified fusion polypeptide comprising SEQ ID NO:22 and another polypeptide and detecting formation of an immunocomplex comprising the fusion polypeptide and the antibody specific for A. actinomycetemcomitans , wherein detection of the immunocomplex indicates the presence of an antibody specific for A. actinomycetemcomitans in the test sample.
11. The method of claim 10 , wherein the test sample is plaque, saliva, crevicular fluid, sputum, blood, serum, plasma, urine, feces, cerebrospinal fluid, amniotic fluid, wound exudate, or tissue.
12. The method of claim 10 , wherein the fusion polypeptide is bound to a solid substrate.
13. The method of claim 10 , wherein the method is an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a western blot assay.