Phospholipase C enzyme(s)
The present invention provides a phospholipase C enzyme(s) having ability to hydrolyze phospholipid in both acidic and around neutral ranges and the activity in a citrate buffer solution as well as having some degree of heat stability, and having a property not to hydrolyze phosphate esters not containing lipid moieties. The phospholipase C enzyme(s) shows the activity at from acidic to neutral pH and does not substantially hydrolyze any phosphate esters except for phospholipids.
1. An isolated DNA consisting of the nucleotide sequence of SEQ ID NO: 4, wherein said isolated DNA encodes a phospholipase C enzyme.
2. An isolated DNA that encodes a phospholipase C enzyme, wherein said isolated DNA comprises the nucleotide sequence of SEQ ID NO: 4.
3. The isolated DNA of claim 1 , wherein the DNA encodes a protein consisting of the amino acid sequence of SEQ ID NO: 5.