Affinity adsorbents for plasminogen
For the separation, removal, isolation, purification, characterization, identification or quantification of plasminogen or a protein that is a plasminogen analogue, an affinity adsorbent is used that is a compound of formula (II) wherein one X is N and the other is N, C—Cl or C—CN; A is a support matrix, optionally linked to the triazine ring by a spacer; Z is O, S or N—R and R is H, C 1-6 alkyl, C 1-6 hydroxyalkyl, benzyl or &bgr;-phenylethyl; B is an optionally substituted hydrocarbon linkage containing from 1 to 10 carbon atoms; D is H, OH or a primary amino, secondary amino, tertiary amino, quaternary ammonium, imidazole, guanidino or amidino group; or B-D is —CHCOOH—(CH 2 ) 3-4 —NH 2 ; and q is 2 to 6.
1. A method for purifying plasminogen or a protein that is a plasminogen analogue, wherein said method comprises contacting a sample containing plasminogen or a plasminogen analogue with an affinity adsorbent of formula II
wherein one X is N and the other X is N, C—Cl or C—CN;
A is a support matrix, optionally linked to the triazine ring by a spacer;
Z is O, S or N—R and R is H, C 1-6 alkyl, C 1-6 hydroxyalkyl, benzyl or β-phenylethyl;
B is an optionally substituted hydrocarbon linkage containing from 1 to 10 carbon atoms;
D is OH or a primary amino; and
q is 2 to 6;
whereby plasminogen or a plasminogen analogue present in the sample binds to the affinity adsorbent of formula II and wherein said method further comprises removing the plasminogen, or analogue thereof, from the affinity adsorbent to obtain plasminogen, or analogue thereof, of greater than 85% purity.
2. The method, according to claim 1 , wherein the adsorbent is of formula III:
3. The method, according to claim 1 , wherein the adsorbent is of formula V:
4. The method, according to claim 1 , wherein the plasminogen is in a sample of plasma.
5. The method, according to claim 1 , wherein said affinity adsorbent is a compound of formula II
wherein one X is N and the other X is N, C—Cl or C—CN;
A is a support matrix, optionally linked to the triazine ring by a spacer;
Z is NH;
B is a hydrocarbon linkage containing from 1 to 10 carbon atoms;
D is OH; and
q is 2 to 6.
6. The method, according to claim 1 , wherein the affinity adsorbent is contacted with a sample of plasma.
7. The method, according to claim 1 , wherein the adsorbent is of formula. IV: