Calcium phosphate delivery vehicles for osteoinductive proteins
View Patent ↗A composition for delivery of osteogenic proteins is disclosed. The composition comprises an osteogenic protein, a calcium phosphate material as a carrier, and an effective amount of an effervescent agent. Methods of making the compositions and methods of using the osteogenic compositions to treat osteoporotic and/or osteopenic bone are also disclosed.
1. A method of treating a mammal having a dental defect comprising administering to the site of the dental defect an effective amount of a composition comprising at least one osteogenic protein, a calcium phosphate material as a carrier, and an effective amount of a material that liberates gas upon dissolution in vivo, wherein the at least one osteogenic protein comprises a member of the TGF-β family.
2. The method of claim 1 , wherein the at least one osteogenic protein comprises a heterodimeric osteogenic protein.
3. The method of claim 1 , wherein the at least one osteogenic protein comprises BMP-2.
4. A method of imbedding an object into a bone of a mammal comprising placing an object comprising non-resorbable hardware into the bone and further securing the object with a composition comprising at least one osteogenic protein, a calcium phosphate material as a carrier, and an effective amount of a material that liberates gas upon dissolution in vivo, wherein the at least one osteogenic protein comprises a member of the TGF-β family.
5. The method of claim 4 , wherein the object is a pin or reinforcing mesh.
6. The method of claim 4 , wherein the object provides an attachment site for a tendon or ligament.
7. The method of claim 4 , wherein the at least one osteogenic protein comprises a heterodimeric osteogenic protein.
8. The method of claim 4 , wherein the at least one osteogenic protein comprises BMP-2.
9. A method of improving biocompatibility of braided sutures comprising coating the braided sutures with a composition comprising at least one osteogenic protein, a calcium phosphate material as a carrier, and an effective amount of a material that liberates gas upon dissolution in vivo, wherein the at least one osteogenic protein comprises a member of the TGF-β family.
10. The method of claim 9 , wherein the braided sutures are dipped in a slurry.
11. The method of claim 9 , further comprising surface treatment of the suture or the composition.
12. The method of claim 9 , wherein surface treatment comprises plasma etching or chemical grafting.
13. The method of claim 9 , wherein the at least one osteogenic protein comprises a heterodimeric osteogenic protein.
14. The method of claim 9 , wherein the at least one osteogenic protein comprises BMP-2.
15. A method of treating a bone defect in a mammal comprising administering to the site of the bone defect an effective amount of a composition comprising at least one osteogenic protein, a calcium phosphate material a carrier, and an effective amount of a material that liberates gas upon dissolution in vivo, wherein the at least one osteogenic protein comprises a member of the TGF-β family.
16. The method of claim 15 , wherein administration of the composition results in a custom fit of the composition to the bone defect.
17. The method of claim 15 , wherein the composition of claim 1 is pre-hardened outside the body, loaded with the biologically active agent and material that liberates gas upon dissolution in vivo, and implanted at a later time.
18. The method of claim 15 , wherein the composition of claim 1 is seeded with bone forming cells.
19. The method of claim 18 , wherein the cells are from an in situ bone lesion.
20. The method of claim 18 , wherein the cells are autologous.
21. The method of claim 18 , wherein the cells are heterologous.
22. The method of claim 15 , wherein the at least one osteogenic protein comprises a heterodimeric osteogenic protein.
23. The method of claim 15 , wherein the at least one osteogenic protein comprises BMP-2.
24. A method of osseous augmentation or regeneration in a mammal comprising administering, to the site where augmentation or regeneration is desired, an effective amount of a composition comprising at least one osteogenic protein, a calcium phosphate material as a carrier, and an effective amount of a material that liberates gas upon dissolution in vivo, wherein the at least one osteogenic protein comprises a member of the TGF-β family.
25. The method of claim 24 , wherein the site of augmentation or regeneration is in osteopenic bone.
26. The method of claim 24 , wherein the augmentation or regeneration is for a bone implant or prosthesis.
27. The method of claim 24 , wherein the at least one osteogenic protein comprises a heterodimeric osteogenic protein.
28. The method of claim 24 , wherein the at least one osteogenic protein comprises BMP-2.