Patent
The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same.
1. An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:2 or a variant having 90% sequence identity.
2. A recombinant nucleic acid comprising:
the isolated nucleic acid of claim 1 ; and
a polynucleotide encoding a protein;
wherein said isolated nucleic acid and said polynucleotide are operably linked such that said isolated nucleic acid causes transcription of said polynucleotide.
3. The recombinant nucleic acid of claim 2 , wherein said protein is a heterologous protein.
4. The recombinant nucleic acid of claim 2 , wherein said protein is an enzyme.
5. The recombinant nucleic acid of claim 2 , wherein said protein is a reporter.
6. The recombinant nucleic acid of claim 2 , wherein said protein is a fusion protein.
7. The recombinant nucleic acid of claim 3 , wherein said protein comprises a signal sequence.
8. A vector comprising the recombinant nucleic acid of claim 2 .
9. A host cell comprising the recombinant nucleic acid of claim 2 .
10. The host cell of claim 9 , wherein said recombinant nucleic acid is present in a genome of said cell.
11. The host cell of claim 9 , wherein said recombinant nucleic acid is present in a vector that replicates in said cell.
12. The host cell of claim 9 , wherein said host cell is a filamentous fungal host cell.
13. A method of producing a protein in a host cell, comprising:
transforming said host cell with the recombinant nucleic acid of claim 3 ;
growing said host cell in a culture medium under conditions in which the protein is produced.
14. The method of claim 13 , further comprising:
recovering said protein from said culture medium.