Microwave accelerated assays
The present invention provides for increasing fluorescence detection in surface assay systems while increasing kinetics of a bioreaction therein by providing low-power microwaves to irradiate metallic materials within the system in an amount sufficient to increase heat thereby affecting the kinetics of a bioreaction therein.
1. An assay detection method comprising:
providing a conductive metallic material, wherein the metallic material is shaped as particles, nanostructures, island or colloids;
introducing at least one biomolecule for disposing near the conductive metallic material, wherein the biomolecule is capable of emitting light and enhanced by a predetermined proximity to the metallic material or the biomolecule is attached to a fluorescent molecule enhanced by a predetermined proximity to the metallic material;
applying microwave energy in the gigahertz frequency range to cause an increase in heat in the metallic material thereby increasing the kinetics of any chemical reactions involving the biomolecule;
applying excitation energy in the IR to UV range to excite the biomolecule or the fluorescent molecule; and
measuring the emitted light from an excited biomolecule or fluorescent molecule.
2. The assay detection method according to claim 1 , wherein the assay is selected from the group comprising immunoassays, hybridization assay, resonance energy transfer assays, polarization/anisotropy based assays, chemiluminescence based assays, luminescence based assays, or enzyme-linked immunosorbent assays.
3. The assay detection method according to claim 1 further comprising:
a conductive metallic material positioned within a container; wherein the metallic material is shaped as particles, nanostructures, island or colloids.
4. The assay detection method according to claim 3 , wherein the metallic material comprises silver or gold.
5. The assay detection method according to claim 3 , wherein the low power microwave energy is from 30 mwatts to 200 watts.
6. The assay detection method according to claim 3 , wherein the metallic material comprises multiple metal particles in the form of islands on a substrate.
7. The assay detection method according to claim 6 , wherein the metallic material comprises multiple metal particles in the form of islands on a substrate and the islands are in triangular forms.
8. The assay detection method according to claim 1 , further comprising providing a spacer attached to the metallic material that binds to the biomolecule to position the fluorescent component of the biomolecule at a predetermined distance from the metallic material to enhance fluorescence intensity of the biomolecule.
9. The assay detection method according to claim 1 , further comprising:
applying a conductive metallic material to a surface used in a detection system, wherein the surface comprises glass, quartz, or a polymeric material.
10. The assay detection method according to claim 9 , wherein the low power microwave energy is from 100 watts to 200 watts.
11. The assay detection method according to claim 9 , wherein the conductive metallic material comprises multiple metal particles in the form of islands on a substrate.
12. The assay detection method according to claim 1 , wherein the biomolecule is a fluorophore containing free capture DNA sequence that is complementary to a known DNA sequence of a targeted pathogen in a sample, wherein the conductive metallic material is immobilized on a surface substrate, wherein the immobilized metallic material has attached thereto an immobilized capture DNA sequence probe that is complementary to the known DNA sequence of the target pathogen, wherein the known DNA sequence of the target pathogen from the sample binds to the immobilized capture DNA sequence; and
irradiating the system with microwave energy in the gigahertz frequency range in an amount sufficient to enhance binding of the fluorophore containing free capture DNA sequence to the known DNA sequence of the target pathogen thereby causing increased speed of the reactions and positioning the fluorophore containing free capture DNA sequence a sufficient distance from the conductive metallic material immobilized on the surface substrate to enhance fluorescence emission of the fluorophore when contacting with excitation energy in the IR to UV range.
13. The assay detection method according to claim 12 , wherein the conductive metallic material comprises metallic particles, nanostructures, islands, or colloids.
14. The assay detection method according to claim 13 , wherein the metallic material is a noble metal.
15. The assay detection method according to claim 13 , wherein the microwave energy is at a power from about 30 mwatts to about 200 watts.
16. The assay detection method according to claim 1 , further comprising a substrate, wherein the substrate is a polymeric well plate used in High Throughput Screening (HTS), the method comprising:
providing a well plate used in HTS systems comprising a multiplicity of wells; and
introducing metallic nanostructures into the wells.