Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;
(b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii); and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
2. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2.
3. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 1.
4. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pTter61F which is contained in E. coli NRRL B-50044.
5. An isolated polynucleotide comprising a nucleotide sequence that encodes the polypeptide of claim 1 .
6. A nucleic acid construct comprising the polynucleotide of claim 5 operably linked to one or more (several) control sequences that direct the production of the polypeptide in an expression host.
7. A recombinant expression vector comprising the nucleic acid construct of claim 6 .
8. A recombinant host cell comprising the nucleic acid construct of claim 6 .
9. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating a cell, which in its wild-type form produces the polypeptide, under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
10. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating a host cell comprising a nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
11. A method of producing a mutant of a parent cell, comprising disrupting or deleting a nucleotide sequence encoding the polypeptide of claim 1 , which results in the mutant producing less of the polypeptide than the parent cell.
12. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating a transgenic plant or a plant cell comprising a polynucleotide encoding the polypeptide under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
13. A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding the polypeptide of claim 1 .
14. A method for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition in the presence of an effective amount of the polypeptide having cellulolytic enhancing activity of claim 1 , wherein the presence of the polypeptide having cellulolytic enhancing activity increases the degradation of cellulose-containing material compared to the absence of the polypeptide having cellulolytic enhancing activity.
15. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulose-containing material with an effective amount of a cellulolytic enzyme composition in the presence of an effective amount of the polypeptide having cellulolytic enhancing activity of claim 1 , wherein the presence of the polypeptide having cellulolytic enhancing activity increases the degradation of cellulose-containing material compared to the absence of the polypeptide having cellulolytic enhancing activity;
(b) fermenting the saccharified cellulose-containing material of step (a) with one or more fermentating microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.
16. The polypeptide of claim 1 , comprising an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.
17. The polypeptide of claim 1 , comprising an amino acid sequence having at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.
18. The polypeptide of claim 1 , comprising an amino acid sequence having at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.
19. The polypeptide of claim 1 , which is encoded by a polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
20. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
21. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
22. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 99% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.