Ultra-nanocrystalline diamond as a biomaterial for enhancing proliferation and differentiation of neural stem cells
View Patent ↗Compositions for promoting growth and/or differentiation of a stem cell are disclosed. The composition comprises: a) a diamond film; b) a stem cell cultured on the diamond film; and c) a medium bathing the stem cell. The stem cell may be a mammalian neural stem cell and the diamond film may comprise a hydrogen-terminated or an oxygen-terminated surface. The hydrogen-terminated surface promotes proliferation and differentiation of a neural stem cell into neurons, and the oxygen-terminated surface promotes a neural stem cell to proliferate and differentiate into oligodendrocytes.
1. A composition comprising:
(a) an ultra-nanocrystalline diamond film, comprising a hydrogen-terminated or an oxygen-terminated surface;
(b) a neural stem cell cultured on the hydrogen-terminated or oxygen-terminated surface of the ultra-nanocrystalline diamond film; and
(c) a medium bathing the neural stem cell.
2. The composition of claim 1 further comprising a neuronal cell and/or an oligodendrocyte differentiated from the neural stem cell, wherein the medium is free of a differentiation agent.
3. The composition of claim 1 , further comprising a neuronal cell differentiated from the neural stem cell, wherein the medium is free of a differentiation agent and the neural stem cell is cultured on the hydrogen-terminated surface of the diamond film.
4. The composition of claim 3 , wherein the ultra-nanocrystalline diamond film comprises the hydrogen-terminated surface.
5. The composition of claim 1 , wherein the stem cell comprises an embryonic neural stem cell.
6. The composition of claim 1 , wherein the stem cell comprises a mammalian neural stem cell.
7. The composition of claim 6 , further comprising a neurosphere derived from the neural stem cell, wherein the ultra-nanocrystalline diamond film comprises the hydrogen-terminated surface.
8. The composition of claim 6 , further comprising a cell differentiated from the neural stem cell.
9. The composition of claim 8 , wherein the cell differentiated from the neural stem cell exhibits a neuronal cell phenotype or an oligodendrocyte cell phenotype.
10. The composition of claim 8 , wherein the medium is free of a differentiation agent.
11. The composition of claim 1 , wherein the ultra-nanocrystalline diamond film comprises the oxygen-terminated surface.
12. An implant material comprising a composition according to claim 1 .
13. An implant material comprising a composition according to claim 8 .
14. The implant material of claim 13 , wherein the diamond film comprises the hydrogen-terminated surface.
15. The implant material of claim 12 , wherein the stem cell is a mammalian neural stem cell.
16. A method for promoting growth and/or differentiation of a neural stem cell, comprising the steps of:
(a) providing a composition according to claim 1 ; and
(b) culturing the neural stem cell on the ultra-nanocrystalline diamond film with the medium for a period and thereby promoting the growth and/or differentiation of the neural stem cell.
17. The method claim 16 , wherein the diamond film comprises the hydrogen-terminated surface.
18. The method claim 16 , wherein the medium is free of a differentiation agent.
19. The method claim 16 , wherein the stem cell is a mammalian neural stem cell.
20. A method for promoting growth and/or differentiation of a neural stem cell, comprising the steps of:
(a) providing a composition according to claim 1 ; and
(b) culturing the neural stem cell on the diamond film with the medium for a period and thereby promoting the growth and/or differentiation of the neural stem cell,
wherein the ultra-nanocrystalline diamond film comprises the hydrogen-terminated surface and the neural stem cell comprises an embryonic neural stem cell.