Compositions against SARS-coronavirus and uses thereof
The present invention provides compositions of binding molecules specifically binding to a coronavirus such as SARS-CoV and capable of neutralizing an infection caused by the virus. The compositions are suitable for diagnosing, preventing and/or treating a condition resulting from a coronavirus such as SARS-CoV.
1. A composition comprising at least two immunoglobulins that are capable of specifically binding to Spike (S) protein of Severe Acute Respiratory Syndrome Coronavirus (SARS-Co-V) and neutralizing SARS-CoV, wherein the first immunoglobulin comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8, and wherein the second immunoglobulin comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10, and wherein the relative amounts of the at least two immunoglobulins are such that they exhibit a synergistic neutralizing activity.
2. The composition of claim 1 , wherein the immunoglobulins are capable of reacting with different, non-competing epitopes of the S protein of SARS-CoV.
3. The composition of claim 2 , wherein the immunoglobulins are capable of reacting with different, non-competing epitopes of amino acids 318-510 of the S protein (SEQ ID NO:115) of SARS-CoV.
4. The composition of claim 1 , wherein at least one of the immunoglobulins is capable of reacting with an animal SARS-like CoV at a region corresponding to amino acids 318-510 of the S protein (SEQ ID NO: 115) of SARS-CoV.
5. The composition of claim 4 , wherein at least one of the immunoglobulins is capable of reacting with amino acids 318-510 of the S protein (SEQ ID NO: 115) to a similar extent as with amino acids 318-510 of the S protein-having a N479K substitution.
6. The composition of claim 1 , wherein each of the immunoglobulins is capable of neutralizing a plurality of SARS-CoV strains.
7. The composition of claim 1 , wherein the two immunoglobulins have a molar ratio of from 1:100 to 100:1.
8. The composition of claim 1 , further comprising a pharmaceutically acceptable excipient.
9. A powder composition for reconstitution, the composition comprising two immunoglobulin molecules that are capable of specifically binding to S protein of SARS-CoV, wherein the immunoglobulin molecule comprising a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 6 and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 10; and the second immunoglobulin molecule comprising a heavy chain variable region comprising the amino acid sequence of SEQ ID NO: 4, and a light chain variable region comprising the amino acid sequence of SEQ ID NO: 8.
10. A method of detecting a Severe Acute Respiratory Syndrome Coronavirus (SARS-Co-V) in a sample, said method comprising the steps of: contacting a sample with a diagnostically effective amount of the immunoglobulins of claim 1 , and determining whether the immunoglobulins specifically binds to a molecule of the sample.
11. The method of claim 10 , wherein the sample is a sample taken from a human subject potentially infected with a SARS-CoV.