Systemic non-nodular endosymbiotic nitrogen fixation in plants
Non-leguminous crops, e.g. wheat, maize and rice, do not form nodules and are dependant for their nutrition on fixed nitrogen from the soil, or from chemical/nitrogenous fertilizers. The present invention provides non-leguminous plants and leguminous plants, including legumes that fail to nodulate with Rhizobia , with bacteria that enable them to fix nitrogen endophytically. Therefore, the plants contain nitrogen fixing bacteria the bacteria being located intracellularly in living plant cells.
1. A seed obtainable from a non-leguminous or leguminous plant containing nitrogen fixing bacteria located in membrane bounded vesicles or vacuoles within the cytoplasm of the plant cells, wherein the bacteria is Acetobacter diazotrophicus , and wherein the bacteria provide fixed nitrogen to said plant; said seed being such that upon germination said bacteria are located in membrane bounded vesicles or vacuoles within the cytoplasm of the living cells of the plant and can provide fixed nitrogen to the plant.