Salicylanilides enhance oral delivery of therapeutic peptides
View Patent ↗This invention pertains to the surprising discovery that salicylanilides, e.g., niclosamide and/or niclosamide analogues when orally administered in conjunction with a peptide pharmaceutical (e.g., a class A amphipathic helical peptide as described herein) significantly increases the bioavailability of that peptide. Methods of peptide delivery using such “delivery agents” and pharmaceutical formulations are provided.
1. A method of mitigating one or more symptoms of atherosclerosis in a mammal, said method comprising:
orally administering to said mammal an effective amount of a “D” or “L” peptide and niclosamide, wherein said peptide ranges in length from about 18 to about 50 amino acids and comprises an amino acid sequence selected from the group consisting of peptide D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F (4F, SEQ ID NO:5), F-A-E-K-F-K-E-A-V-K-D-Y-F-A-K-F-W-D (retro 4F, SEQ ID NO: 104), D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F (6F, SEQ ID NO:7), and F-F-E-K-F-K-E-F-F-K-D-Y-F-A-K-L-W-D (retro 6F, SEQ ID NO:537).
2. The method of claim 1 , wherein said niclosamide is administered before said peptide.
3. The method of claim 1 , wherein said niclosamide is administered simultaneously with peptide.
4. The method of claim 1 , wherein said niclosamide and said peptide are administered as a single formulation.
5. The method of claim 1 , wherein said niclosamide and said peptide are combined to form an adduct prior to administration.
6. The method of claim 1 , wherein all the amino acids of said peptide are “D” amino acids.
7. The method of claim 1 , wherein all the amino acids of said peptide are “L” amino acids.
8. The method of claim 1 , wherein said peptide comprises a protecting group at the amino or carboxyl terminus.
9. The method of claim 8 , wherein said protecting group is a protecting group selected from the group consisting of acetyl, amide, and 3 to 20 carbon alkyl groups, Fmoc, Tboc, 9-fluoreneacetyl group, 1-fluorenecarboxylic group, 9-florenecarboxylic group, 9-fluorenone-1-carboxylic group, benzyloxycarbonyl, Xanthyl (Xan), Trityl (Trt), 4-methyltrityl (Mtt), 4-methoxytrityl (Mmt), 4-methoxy-2,3,6-trimethyl-benzenesulphonyl (Mtr), Mesitylene-2-sulphonyl (Mts), 4,4-dimethoxybenzhydryl (Mbh), Tosyl (Tos), 2,2,5,7,8-pentamethyl chroman-6-sulphonyl (Pmc), 4-methylbenzyl (MeBzl), 4-methoxybenzyl (MeOBzl), Benzyloxy (BzlO), Benzyl (Bzl), Benzoyl (Bz), 3-nitro-2-pyridinesulphenyl (Npys), 1-(4,4-dimentyl-2,6-diaxocyclohexylidene)ethyl (Dde), 2,6-dichlorobenzyl (2,6-DiCl-Bzl), 2-chlorobenzyloxycarbonyl (2-Cl—Z), 2-bromobenzyloxycarbonyl (2-Br—Z), Benzyloxymethyl (Bom), t-butoxycarbonyl (Boc), cyclohexyloxy (cHxO),t-butoxymethyl (Bum), t-butoxy (tBuO), t-Butyl (tBu), Acetyl (Ac), and Trifluoroacetyl (TFA).
10. The method of claim 8 , wherein said peptide comprises a first protecting attached to the amino terminus and a second protecting group attached to the carboxyl terminus.
11. The method of claim 10 , wherein said first protecting group is a protecting group selected from the group consisting of acetyl, propionyl, and a 3 to 20 carbon alkyl.
12. The method of claim 10 , wherein said second protecting group is an amide.
13. The method of claim 1 , wherein said niclosamide and said peptide are administered as a unit dosage formulation.
14. The method of claim 1 , wherein said niclosamide and said peptide are administered as a unit dosage formulation formulated so that the niclosamide is released or solubilized before the peptide.
15. The method of claim 1 , wherein said peptide has a formula selected from the group consisting of Ac-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH 2 (4F, SEQ ID NO:5), Ac-F-A-E-K-F-K-E-A-V-K-D-Y-F-A-K-F-W-D-NH 2 (retro 4F, SEQ ID NO: 104), Ac-D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F-NH 2 (6F, SEQ ID NO:7), and Ac-F-F-E-K-F-K-E-F-F-K-D-Y-F-A-K-L-W-D-NH 2 (retro 6F, SEQ ID NO:537).
16. The method of claim 1 , wherein said peptide consists of all “L” amino acids and the amino acid sequence of said peptide consists of the sequence D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F (SEQ ID NO:7).
17. The method of claim 16 , wherein said peptide has the formula: Ac-D-W-L-K-A-F-Y-D-K-F-F-E-K-F-K-E-F-F-NH 2 (6F, SEQ ID NO:7).
18. The method of claim 1 , wherein said peptide consists of all “L” amino acids and the amino acid sequence of said peptide consists of the sequence F-F-E-K-F-K-E-F-F-K-D-Y-F-A-K-L-W-D (SEQ ID NO:537).
19. The method of claim 18 , wherein said peptide has the formula Ac-F-F-E-K-F-K-E-F-F-K-D-Y-F-A-K-L-W-D-NH 2 (SEQ ID NO:537).
20. The method of claim 1 , wherein said peptide consists of all “L” amino acids and the amino acid sequence of said peptide consists of the sequence D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F (SEQ ID NO:5).
21. The method of claim 1 , wherein said peptide has the formula Ac-D-W-F-K-A-F-Y-D-K-V-A-E-K-F-K-E-A-F-NH 2 (SEQ ID NO:5).
22. The method of claim 1 , wherein said peptide consists of all “L” amino acids and the amino acid sequence of said peptide consists of the sequence F-A-E-K-F-K-E-A-V-K-D-Y-F-A-K-F-W-D (SEQ ID NO: 104).
23. The method of claim 22 , wherein said peptide has the formula Ac-F-A-E-K-F-K-E-A-V-K-D-Y-F-A-K-F-W-D-NH 2 (SEQ ID NO: 104).