Association of EDG5 polymorphism V286A with type II diabetes mellitus and venous thrombosis/pulmonary embolism and the use thereof
The present invention relates to a method of identifying an increase in risk for type II Diabetes mellitus, venous thrombosis, or pulmonary embolism in a subject, wherein the presence of an amino acid exchange at position 286 from valine (Val) to alanine (Ala) in the EDG5 protein in a biological sample taken from the subject.
1. A method for identifying an increase risk for venous thrombosis, pulmonary embolism, or a combination thereof in a human subject, comprising the steps of:
(a) removing a biological sample from the human subject that comprises a nucleic acid sequence that encodes EDG5 protein;
(b) screening genomic DNA of said human subject for single polynucleotide polymorphism (SNP) by polymerase chain reaction amplification (PCR) of DNA encoding position 286 of SEQ ID NO:3 of EDG5 protein; and
(c) detecting the presence of a nucleotide sequence that encodes for an alanine at position 286 of SEQ ID NO. 3 and determining the presence of the nucleotide sequence is correlated with an increased risk for venous thrombosis, pulmonary embolism, or a combination thereof in said human subject.