Interleukin-10 antibodies
The methods and compositions provided herein relate generally to IL-10 specific antibodies and uses thereof. More specifically, compositions of humanized IL-10 specific antibodies and methods to use such antibodies in modulating the biological activity of IL-10, particularly in autoimmune disorders and pathogen-mediated immunopathology.
1. A humanized recombinant antibody molecule that binds IL-10, or binding fragment thereof, comprising:
at least one antibody light chain, or binding fragment thereof, said light chain or said binding fragment thereof comprising SEQ ID NO:11 at CDR1, SEQ ID NO:12 at CDR2, and SEQ ID NO:13 at CDR3; and a framework region, wherein the amino acid sequence of framework region is all or substantially all of a human immunoglobin amino acid sequence; and
at least one antibody heavy chain, or binding fragment thereof, said heavy chain or said binding fragment thereof comprising SEQ ID NO:15 at CDR1, SEQ ID NO:16 at CDR2, and SEQ ID NO:17 at CDR3; and a framework region, wherein the amino acid sequence of framework region is all or substantially all of a human immunoglobin amino acid sequence.
2. The antibody of claim 1 , further comprising a heavy chain constant region, wherein the heavy chain constant region comprises a γ1, γ2, γ3, or γ4 human heavy chain constant region or a variant thereof.
3. The antibody of claim 1 , further comprising a light chain constant region, wherein the light chain constant region comprises a lambda or a kappa human light chain constant region.
4. The antibody of claim 1 , wherein the binding fragment is an antibody fragment selected from the group consisting of Fab, Fab′, Fab′-SH, Fv, scFv, F(ab′) 2 , and a diabody.
5. The antibody of claim 1 , wherein the antibody light chain, or binding fragment thereof, comprises a polypeptide having a variable region and a constant region of SEQ ID NO:14.
6. The antibody of claim 1 , wherein the antibody heavy chain, or binding fragment thereof, comprises a polypeptide having a variable region and a constant region of SEQ ID NO:18.
7. A chimeric recombinant antibody molecule that binds IL-10, or binding fragment thereof, comprising:
at least one antibody light chain, or binding fragment thereof, said light chain or said binding fragment thereof comprising SEQ ID NO:11 at CDR1, SEQ ID NO:12 at CDR2, and SEQ ID NO:13 at CDR3; and
at least one antibody heavy chain, or binding fragment thereof, said heavy chain or said binding fragment thereof comprising SEQ ID NO:15 at CDR1, SEQ ID NO:16 at CDR2, and SEQ ID NO:17 at CDR3.
8. A composition comprising the antibody, or binding fragment thereof of claim 1 or 7 in combination with a pharmaceutically acceptable carrier or diluent.
9. The composition of claim 8 , further comprising an immunosuppressive agent.