Adsorbents comprising anthraquinone dye-ligends for the separation of biological materials
A process for the separation of biological materials, such as dye-ligand affinity chromatography, wherein an adsorbent is used, which comprises a reaction product of certain reactive anthroquinone compounds and a substrate having a group capable of reaction with a reactive group in said reactive anthroquinone compounds to form a covalent bond.
1. A process for the separation of biological materials, which process comprises treating or contacting biological materials with an adsorbent comprising a reaction product of a compound of formula (3), (4) or (5)
wherein
V 1 is β-hydroxyethylamino, 2-(β-hydroxyethoxy)ethylamino or morpholino,
V 2 and V 3 each independently of the other are β-hydroxyethylamino, 2-(β-hydroxyethoxy)ethylamino, N,N-di-β-hydroxyethlamino or morpholino and
q is the number 0, 1, 2 or 3
and a substrate having a group capable of reaction with a reactive group in said compound of formula (3), (4) or (5) to form a covalent bond.
2. A process according to claim 1 , wherein
the compound of formula (3), (4) or (5) is selected from the group consisting of (3 a ), (3 b ), (3 c ), (3 d ), (3 e ), (3 f ), (4 a ), (4 b ), (4 c ), (4 d ), (4 e), ( 4 f ), (4 q ), (4 h ), (5 a ), (5 b ), (5 c ) and (5 d )
3. A process according to claim 1 , wherein the substrate is a carbohydrate or a modified carbohydrate.
4. Adsorbent for the separation of biological materials, which comprises a reaction product of a compound of formula (3), (4) or (5)
wherein
V 1 is β-hydroxyethylamino, 2-(βhydroxyethoxy)ethylamino or morpholino,
V 2 and V 3 each independently of the other are β-hydroxyethylaminoe, 2(β-hydroxyethoxy)ethylamino, N,N-di-β-hydroxyethylamino or morpholino and
q is the number 0, 1, 2 or 3
and a substrate having a group capable of reaction with a reactive group in said compound of formula (3), (4) or (5) to form a covalent bond.
5. A process for the preparation of an adsorbent, which process comprises reacting a compound of formula (3), (4) or (5)
wherein
V 1 is β-hydroxyethylamino, 2-(βhydroxyethoxy)ethylamino or morpholino,
V 2 and V 3 each independently of the other are β-hydroxyethylamino, 2(β-hydroxyethoxy)ethylamino, N,N-di-β-hydroxyethylamino or morpholino and
q is the number 0, 1, 2 or 3
with a substrate having a group capable of reaction with a reactive group in said compound of formula (3), (4) or (5) to form a covalent bond in the presence of an acid binding agent.
6. Compounds of formulae (3), (4) or (5)
wherein
V 1 is β-hydroxyethylamino, 2-(βhydroxyethoxy)ethylamino or morpholino,
V 2 and V 3 each independently of the other are β-hydroxyethylamino, 2(β-hydroxyethoxy)ethylamino, N,N-di-β-hydroxyethylamino or morpholino and
q is the number 0, 1, 2 or 3.
7. A process according to claim 3 , wherein the substrate is a carbohydrate or a modified carbohydrate selected from agarose, cross-linked agarose, dextrose, dextrans and modified versions thereof.