Detection of penicillin tolerance in Group B
Disclosed are methods of detecting penicillin tolerance in Group B Streptococcus by detecting at least one of two single nucleotide polymorphisms (SNP) in penicillin binding protein 4. Also disclosed are primers and hybridization probes that may be used in such methods.
1. A method of detecting penicillin tolerance in Group B Streptococcus , comprising:
(a) providing a biological sample; and
(b) detecting at least one single nucleotide polymorphism (SNP) in penicillin binding protein 4 in said biological sample, wherein said SNP is selected from the group consisting of G503A and G865A, and wherein the presence of said SNP is indicative of penicillin tolerance in Group B Streptococcus.
2. The method of claim 1 , wherein said biological sample is selected from the group consisting of a cervicovaginal swab, rectal swab, whole blood, urine, cerebrospinal fluid, and ascites fluid.
3. The method of claim 1 , wherein the detecting step is performed by a PCR technology selected from the group consisting of real-time PCR, pyrosequencing, and conventional PCR followed by sequencing.
4. The method of claim 1 , wherein the detecting step is performed by real-time PCR.
5. The method of claim 4 , wherein said real-time PCR is performed using a primer set comprising:
(a) at least one forward primer selected from the group consisting of a nucleotide sequence of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO:17; and
(b) at least one reverse primer selected from the group consisting of a nucleotide sequence of SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, and SEQ ID NO: 18.
6. The method of claim 4 , wherein said real-time PCR is performed using at least one isolated hybridization probe comprising at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, and SEQ ID NO: 19.
7. The method of claim 4 , wherein said real-time PCR is performed at an annealing temperature of about 58° C. to about 60° C.
8. The method of claim 4 , wherein said real-time PCR is performed with an annealing time of about 30 seconds to about 60 seconds.
9. The method of claim 4 , wherein said detecting step utilizes molecular beacon technology with a specific primer set to detect at least one SNP in penicillin binding protein 4 selected from the group consisting of G503A and G865A.
10. The method of claim 1 , wherein said detecting step is performed by pyrosequencing.
11. The method of claim 1 , wherein said detecting step comprises conventional PCR followed by sequencing.
12. The method of claim 11 , wherein said conventional PCR is performed using a primer set, wherein said primer set is specific for at least one single nucleotide polymorphism in penicillin binding protein 4 selected from the group consisting of G503A and G865A.
13. The method of claim 12 , wherein said primer set specific for G503A comprising:
(a) at least one forward primer having a nucleotide sequence of SEQ ID NO: 1; and
(b) at least one reverse primer having a nucleotide sequence of SEQ ID NO: 3.
14. The method of claim 12 , wherein said primer set specific for G865A comprising:
(a) at least one forward primer having a nucleotide sequence of SEQ ID NO: 2; and
(b) at least one reverse primer having a nucleotide sequence of SEQ ID NO: 4.
15. The method of claim 1 , further comprising (c) detecting Group B Streptococcus using at least one primer set against a Group B Streptococcus -specific gene, wherein presence of said Streptococcus -specific gene is indicative of the presence of Group B Streptococcus.
16. The method of claim 15 , wherein said detecting step (b) and said detecting step (c) are performed substantially simultaneously.
17. The method of claim 15 , wherein said detecting step (b) and said detecting step (c) are performed sequentially.
18. The method of claim 15 , wherein said Group B Streptococcus -specific gene is CAMP factor.
19. A set of isolated oligonucleotides comprising a forward primer, a reverse primer and a hybridization probe, wherein said forward primer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4, said reverse primer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 17, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, and SEQ ID NO: 18, and said hybridization probe having a nucleotide sequence selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, and SEQ ID NO: 19.
20. A method of diagnosing penicillin tolerance in a mammal, comprising:
(a) providing a biological sample from a mammal;
(b) detecting at least one single nucleotide polymorphism (SNP) in penicillin binding protein 4 in said biological sample, wherein said SNP is selected from the group consisting of G503A and G865A; and
(c) correlating presence of said SNP to penicillin tolerance in Group B Streptococcus.
21. The method of claim 20 , wherein the mammal is a human.
22. The method of claim 20 , wherein if said mammal is diagnosed positive for penicillin tolerance, the method further comprises administering a non-penicillin antibiotic to said mammal.
23. A kit for detecting penicillin tolerance in Group B Streptococcus , comprising:
(a) at least one primer set capable of detecting at least one single nucleotide polymorphism (SNP) in penicillin binding protein 4 in a biological sample, wherein said SNP is selected from the group consisting of G503A and G865A;
b) at least one hybridization probe capable of detecting at least one SNP in penicillin binding protein 4 in a biological sample, wherein said SNP is selected from the group consisting of G503A and G865A; and
c) an instruction for using said primer set to detect said at least one SNP.
24. The kit of claim 23 , wherein said instruction provides guidance to use said primer set and said hybridization probe in performing real-time PCR in detecting penicillin tolerance in Group B Streptococcus in a biological sample.
25. The kit of claim 23 , wherein said primer set contains a forward primer and a reverse primer, wherein said forward primer having a nucleotide sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 17, and wherein said reverse primer having a nucleotide sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and SEQ ID NO:18.
26. The kit of claim 23 , wherein said instruction provides guidance to use said primer set in performing conventional PCR followed by sequencing in detecting penicillin tolerance in Group B Streptococcus in a biological sample.
27. The kit of claim 23 , wherein said primer set contains a forward primer and a reverse primer, wherein said forward primer having a nucleotide sequence selected from the group consisting of SEQ ID NO:1, and SEQ ID NO: 2, and wherein said reverse primer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 3, and SEQ ID NO: 4.