Dendritic cell precursors
A method of generating a population of dendritic cell (DC) precursors includes obtaining a population of progenitor cells from a subject and culturing the progenitor cells in a culture medium. The culture medium can include Flt3 ligand and interleukin-6 and be free of granulocyte-macrophage colony-stimulating factor.
1. A method of generating a population of dendritic cell (DC) precursors, the method comprising the steps of:
obtaining bone marrow from the subject, the bone marrow including CD34 pos progenitor cells from a subject;
culturing the progenitor cells in a culture medium including Flt3 ligand (Flt3-L), interleukin-6 (IL-6), and a STAT5 antagonist, and being free of granulocyte-macrophage colony-stimulating factor (GMCSF), the progenitor cells being cultured for a time and under conditions effective to allow the progenitor cells to differentiate into DC precursors having a surface marker phenotype B220 pos CD11c neg /MHC Class II neg ; and
selecting the B220 pos /CD11c neg /MHC Class II neg DC precursors from the culture.
2. The method of claim 1 , the progenitor cells being cultured for a time and under conditions sufficient to allow the progenitor cells to differentiate into DC precursors having a surface marker phenotype B220 pos /CD11c neg /MHC Class II neg CD45RA pos .
3. The method of claim 1 , wherein the culture medium further comprises SCF.
4. A method of generating a population of dendritic cell (DC) precursors, the method comprising the steps of:
obtaining CD34 pos progenitor cells from a subject;
culturing the progenitor cells in a culture medium that includes Flt3 ligand (Flt3-L), interleukin-6 (IL-6), and a STAT5 antagonist, and being free of granulocyte-macrophage colony-stimulating factor (GMCSF), the progenitor cells being cultured in the culture medium for a time and under conditions effective to allow the progenitor cells to differentiate into DC precursors having a surface marker phenotype B220 pos /CD11c neg /MHC Class II neg ; and
selecting the B220 pos /CD11c neg /MHC Class II neg DC precursors from the culture.
5. The method of claim 4 , the progenitor cells being cultured for a time and under conditions sufficient to allow the progenitor cells to differentiate into DC precursors having a surface marker phenotype B220 pos /CD11c neg /MHC Class II neg /CD45RA Pos .
6. The method of claim 4 , the culture medium further including SCF.
7. The method of claim 4 , the progenitors cells being isolated from bone marrow of the subject.