Restricted access media and methods for making restricted access media
View Patent ↗The present invention is directed to restricted access media (RAM), methods for preparing restricted access media, and kits for preparing restricted access media that contain protected ligand binding agents or protected enzymes. Certain RAM provided contain a plurality of protected regions of the support that contain ligand binding agents that are protected by blocking agents. Certain RAM provided contain a plurality of protected regions of the support that contain unbound ligand binding agents or enzymes that are retained in the protected regions by a capping agent. Methods of making the RAM of the invention and associated kits are also provided.
1. A restricted access media comprising a support wherein a plurality of protected regions of said support contain one or more unbound ligand binding agent(s) or enzyme molecules, and wherein said unbound ligand binding agent(s) or enzyme molecule(s) are retained in said protected regions by a capping agent that is bound to said support, and wherein said ligand binding agent(s) or enzyme molecule(s) are not bound to said support.
2. The restricted access media of claim 1 , wherein said support comprises an inorganic material, a biological material, an organic material, an organic polymer, a composite support, or a modified support.
3. The restricted access media of claim 1 , wherein said unbound ligand binding agent comprises a protein, a glycoprotein, a DNA, a RNA, a nucleoprotein, a carbohydrate containing agent, a lipoprotein, or a combination thereof.
4. The restricted access media of claim 1 , wherein said capping agent comprises a glycoprotein, a glycolipid, a carbohydrate containing agent, or a combination thereof.
5. The restricted access media of claim 4 , wherein said carbohydrate containing agent comprises glycogen, dextran, agarose, or cellulose.
6. A method for preparing a restricted access media comprising:
a) incubating an activated support with a solution comprising: i) a ligand binding agent or an enzyme molecule and ii) an activated capping agent; and
b) removing unbound capping agent from said incubated support, thereby preparing the restricted access media comprising a support wherein a plurality of protected regions of said support contain one or more unbound ligand binding agent(s) or enzyme molecule(s) 1 and wherein said unbound ligand binding agent(s) or enzyme molecule(s) are retained in said protected regions by said capping agent that is bound to said support, and wherein said ligand binding agent(s) or enzyme molecule(s) are not bound to said support.
7. The method of claim 6 , wherein said ligand binding agent or said enzyme molecule and said activated capping agent are incubated together or separately with said activated support.
8. The method of claim 6 , wherein said activated support is activated by a hydrazide group, an amine group, an aldehyde group, or a combination thereof.
9. The method of claim 6 , wherein said unbound ligand binding agent or enzyme molecule comprises a protein, a glycoprotein, a DNA, a RNA, a nucleoprotein, a carbohydrate containing agent, a lipoprotein, or a combination thereof.
10. The method of claim 6 , wherein said support comprises a porous support with a pore size of about 50 Å to about 1000 Å.
11. A kit comprising a restricted access media that comprises comprising: i) a support wherein a plurality of protected regions of said support contain one or more unbound ligand binding agent or enzyme molecules, and wherein said ligand binding agent(s) or enzyme molecules are retained in said protected regions by a capping agent that is bound to said support, and wherein said ligand binding agent(s) or enzyme molecule(s) are not bound to said support, and ii) at least one container.