BGL6 β-glucosidase and nucleic acids encoding the same
The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.
1. A method of producing ethanol, said method comprising the steps of:
a) contacting a biomass composition with an enzymatic composition comprising a β-glucosidase 6 having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2 to yield a sugar solution;
b) adding to the sugar solution a fermentative microorganism; and
c) culturing the fermentative microorganism under conditions sufficient to produce ethanol,
wherein the biomass composition may be optionally pretreated.
2. The method of claim 1 wherein step (a) further comprises the addition of at least one endoglucanase.
3. The method of claim 1 wherein step (a) further comprises the addition of at least one cellbiohydrolase.
4. The method of claim 2 wherein step (a) further comprises the addition of at least one cellbiohydrolase.
5. The method of claim 1 wherein the pretreatment is with a dilute acid.
6. A method of producing ethanol, said method comprising the steps of:
a) contacting a biomass composition with an enzymatic composition comprising a β-glucosidase having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2 and a fermentative microorganism; and
b) culturing the fermentative microorganism under conditions sufficient to produce ethanol,
wherein the biomass composition may be optionally pretreated.
7. The method of claim 6 wherein step (a) further comprises the addition of at least one endoglucanase.
8. The method of claim 6 wherein step (a) further comprises the addition of at least one cellbiohydrolase.
9. The method of claim 7 wherein step (a) further comprises the addition of at least one cellbiohydrolase.
10. The method of claim 6 wherein the pretreatment is with a dilute acid.
11. The method of claim 1 or 6 wherein the β-glucosidase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2.