Recombinant fragments and synthetic peptides of 17-kDa polypeptide useful in detecting
The present disclosure describes recombinant and synthetic polypeptides of Bartonella henselae and related methods and kits for the detection of antibodies specific for Bartonella henselae . The 17-kDa polypeptides, methods and kits are useful in the detection of recent and/or ongoing infections with Bartonella henselae , which can be useful in the diagnosis of Cat Scratch Disease (CSD).
1. A synthetic polypeptide consisting of the amino acid sequence SEQ ID NO: 22, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
2. A synthetic polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26 and SEQ ID NO: 27, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
3. A synthetic polypeptide consisting of the amino acid sequence SEQ ID NO: 32, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
4. A synthetic polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO: 30 and SEQ ID NO: 31, whereby said synthetic polypeptide, when assayed in an ELISA assay, reacts to IFA sero-positive sera and does not react to IFA sero-negative sera from a patient infected with Bartonella henselae.
5. A kit useful in the detection of Bartonella henselae , said kit comprising:
(a) the synthetic polypeptide according to claim 1 ; and
(b) an instruction for using said synthetic polypeptide in an ELISA assay.
6. A kit useful in the detection of Bartonella henselae , said kit comprising:
(a) the synthetic polypeptide according to claim 2 ; and
(b) an instruction for using said synthetic polypeptide in an ELISA assay.
7. A kit useful in the detection of Bartonella henselae , said kit comprising:
(a) the synthetic polypeptide according to claim 3 ; and
(b) an instruction for using said synthetic polypeptide in an ELISA assay.
8. A kit useful in the detection of Bartonella henselae , said kit comprising:
(a) the synthetic polypeptide according to claim 4 ; and
(b) an instruction for using said synthetic polypeptide in an ELISA assay.
9. A method for detection of Bartonella henselae in a biological sample from a mammal, comprising the steps of:
(a) immobilizing a synthetic polypeptide to a solid support, wherein said synthetic polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 30, SEQ ID NO: 31, and SEQ ID NO. 32;
(b) adding a biological sample onto said support to permit antibodies present in said biological sample to bind to said immobilized synthetic polypeptide;
(c) washing to remove any unbound antibodies; and
(d) detecting bound antibodies, wherein the presence of said bound antibodies is indicative of infection of said mammal with Bartonella hensela.
10. The method according to claim 9 , wherein said mammal is a human.