Immunogenic substances comprising a polyinosinic acid—polycytidilic acid based adjuvant
The present invention provides a polynucleotide adjuvant (PICKCa) composition and methods of use in eliciting an immune response, in particular a mucosal immune response. The polynucleotide adjuvant comprises of a polyriboinosinic-polyribocytidylic acid (PIC), at least one antibiotic and at least one positive ion. The present invention also provides an immunogenic composition comprising the polynucleotide adjuvant composition together with other immunogenic compositions such as an antigen (e.g., as in a vaccine) selected from viral, bacterial, fungal, parasitic and/or cancer antigens. The present invention further contemplates methods of use of such adjuvant compositions, particularly in eliciting an immune response, in particular a mucosal immune response to an antigenic compound.
1. An immunogenic composition suitable for human use, the immunogenic composition comprising:
(a) a polynucleotide adjuvant composition comprising complexes of a polyriboinosinic-polyribocytidylic acid (PIC), kanamycin, and calcium, wherein the polynucleotide adjuvant composition comprises said complexes having sedimentation coefficients greater than 9.0 Svedbergs; and
(b) at least one antigen.
2. The immunogenic composition according to claim 1 , wherein the polynucleotide adjuvant composition comprises said complexes having sedimentation coefficients greater than 12.0 Svedbergs.
3. The immunogenic composition according to claim 1 , wherein the antigen is an inactivated microorganism, attenuated microorganism, recombinant polypeptide, synthetic polypeptide, attenuated toxin, protein subunit, polysaccharide conjugate, tumor antigen, or recombinant DNA.
4. The immunogenic composition according to claim 1 , wherein the antigen is a viral antigen.
5. The immunogenic composition according to claim 4 , wherein the viral antigen is an antigen of adeniviridae, arenaviridae, astroviridae, bunyaviridae, caliciviridae, coronaviridae, flaviviridae, hepadnaviridae, hepatitis delta virus, hepeviridae, herpesviridae, mononegavirales, nidovirales, picornaviridae, orthomyxoviridae, papillomaviridae, paramyxoviridae, parvoviridae, polyomaviridae, poxviridae, rhabdoviridae, reoviridae, retroviridae or togaviridae.
6. The immunogenic composition according to claim 1 , wherein the antigen is a bacterial antigen.
7. The immunogenic composition according to claim 6 , wherein the bacterial antigen is an antigen of actinobacteria, chlamydiae, firmicutes, proteobacteria, or spirochaetes.
8. The immunogenic composition according to claim 1 , wherein the antigen is a fungal antigen.
9. The immunogenic composition according to claim 8 , wherein the fungal antigen is an antigen of ascomycota or basidiomycota.
10. The immunogenic composition according to claim 1 , wherein the antigen is a parasitic antigen.
11. The immunogenic composition according to claim 10 , wherein the parasitic antigen is an antigen of phylum sarcomastigophora, phuylum apiicomplexa, phylum ciliophora, phylum plathyhelminthes, phylum nematoda or phylum arthropoda.
12. The immunogenic composition according to claim 1 , wherein the antigen is a cancer antigen.
13. The immunogenic composition according to claim 12 , wherein the cancer antigen is an antigen of bone, brain, breast, digestive/gastrointestinal, endocrine, eye, genitourinary, germ cell, gynecologic, head and neck, hematological/blood, lung, musculoskeletal, neurologic, respiratory/thoracic or skin cancer.
14. The immunogenic composition according to claim 1 , wherein the composition comprises two or more antigens.
15. The immunogenic composition according to claim 1 , further comprising at least one immunomodulator.
16. A kit comprising the immunogenic composition of claim 1 .
17. A method for eliciting an immune response to an antigen in a host, the method comprising:
administering to the host the immunogenic composition of claim 1 .
18. The method according to claim 17 , wherein the host has an infectious disease and said antigen is from the pathogen causing the infectious disease.
19. The method according to claim 17 , wherein said administering comprises parenteral injection, intramuscular injection, intraperitoneal injection, intravenous injection, subcutaneous injection, intranasal delivery, topical delivery, transdermal delivery or intradermal delivery.