Expression of granular starch hydrolyzing enzymes in

1. A process for producing an amino acid end product from a granular starch slurry, said process comprising:

contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a glucoamylase, wherein the glucoamylase has at least 97% amino acid sequence identity with SEQ ID NO:3, at a temperature equal to or below the gelatinization temperature of the granular starch;

allowing the alpha amylase and the glucoamylase to act for a period of time sufficient to hydrolyze the granular starch comprising to obtain glucose;

contacting the glucose with a microorganism capable of converting the glucose to an amino acid end product; and,

producing an amino acid end product from the granular starch slurry.

2. The process according to claim 1 wherein the contacting comprises simultaneously hydrolyzing the granular starch in a reaction mixture comprising the microorganism.

3. The process according to claim 1 wherein the granular starch substrate is obtained from corn, wheat, barley, or rice.

4. The process according to claim 3 wherein the granular starch substrate is corn starch obtained from whole grain.

5. The process according to claim 3 wherein the granular starch substrate is dry milled or wet milled.

6. The process according to claim 3 wherein the dry solids content is 15%-55%.

7. The process according to claim 3 wherein the contacting step is conducted at a pH of 5.0 to 6.0.

8. The process according to claim 3 wherein the alpha amylase is derived from a Bacillus.