DNA encoding glyceroglycolipid lipase
A glyceroglycolipid lipase which is highly safe, hydrolyzes a neutral fat, a glycerophospholipid or a glyceroglycolipid at about pH 6, is thermally stable, hydrolyzes lecithin, does not hydrolyze lysolecithin, can rise a bread when used singly in the production of the bread, and has no unpleasant odor. Specifically disclosed is a glyceroglycolipid lipase derived from a filamentous bacterium Aspergillus japonicus.
1. An isolated DNA selected from the group consisting of:
a) a DNA consisting of the nucleotide sequence from nucleotide 110 to nucleotide 991 of SEQ ID NO: 1;
b) a DNA comprising a nucleotide sequence at least 95% identical to SEQ ID NO:1 that encodes a protein having a glyceroglycolipid degradation activity;
c) a DNA that remains hybridized to a nucleotide sequence consisting of nucleotides 110 to 991 of SEQ ID NO: 1 under stringent conditions which comprise washing hybridized DNA at 60° C. in 0.1×SSC, and wherein said DNA encodes a protein having a glyceroglycolipid degradation activity;
d) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO: 2 or a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 2, wherein the protein has glyceroglycolipid degradation activity; and
e) a DNA comprising the nucleotide sequence of nucleotides 110 to 991 of SEQ ID NO: 1.
2. The isolated DNA of claim 1 that consists of the nucleotide sequence from nucleotide 110 to nucleotide 991 of SEQ ID NO: 1.
3. The isolated DNA of claim 1 that comprises a nucleotide sequence at least 95% identical to the nucleotide sequence of nucleotides 110 to 991 of SEQ ID NO: 1 and that encodes a protein having a glyceroglycolipid degradation activity.
4. The isolated DNA of claim 1 , wherein the DNA remains hybridized to a DNA consisting of the nucleotide sequence of nucleotides 110 to 991 of SEQ ID NO: 1 under stringent conditions that comprise washing hybridized DNA at 60° C. in 0.1×SSC, and that encodes a protein having a glyceroglycolipid degradation activity.
5. The isolated DNA of claim 1 that encodes a protein comprising the amino acid sequence of SEQ ID NO: 2 or a protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 2, wherein the protein has glyceroglycolipid degradation activity.
6. The isolated DNA of claim 1 that comprises the nucleotide sequence from nucleotides 110 to 991 of SEQ ID NO: 1.
7. A vector comprising the isolated DNA of claim 1 .
8. The vector of claim 7 that is a vector for a prokaryotic cell.
9. The vector of claim 7 that is a vector for a eukaryotic cell.
10. A host cell transformed with the vector of claim 7 .
11. A prokaryotic cell transformed with the vector of claim 8 .
12. A eukaryotic cell transformed with the vector of claim 9 .
13. A method for making a glyceroglycolipase encoded by the isolated DNA of claim 1 , comprising expressing a host cell containing said isolated DNA under conditions suitable for expression of said glyceroglycolipase.
14. The method of claim 13 , wherein the host cell is a prokaryote.
15. The method of claim 13 , wherein the host cell is a eukaryote.