Multimeric oxidoreductases
Described are multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate. The complexes having a dehydrogenase subunit and a cytochrome C subunit. Also described are polynucleotides coding for the multimeric complexes and methods of use thereof.
1. An isolated polynucleotide coding for a multimeric oxidoreductase complex, said complex having oxidoreductase activity and comprising a cytochrome C subunit comprising a sequence having at least 95% sequence identity to SEQ ID NO:5 and a dehydrogenase subunit comprising a sequence having at least 95% sequence identity to SEQ ID NO:23.
2. The isolated polynucleotide of claim 1 , wherein the polynucleotide includes a third coding region for the multimeric complex comprising a sequence having at least 95% sequence identity to SEQ ID NO:41.
3. A vector comprising the polynucleotide of claim 1 .
4. A vector comprising the polynucleotide of claim 2 .
5. A bacterial host cell transformed with a vector of claim 3 .
6. A bacterial host cell transformed with a vector of claim 4 .
7. The bacterial host cell of claim 5 , wherein the bacterial host cell is a Enterobacteriaceae strain, optionally a Pantoea, Klebsiella, Erwinia or E. coli strain or a Pseudomonadacea strain.
8. The bacterial host cell of claim 6 , wherein the bacterial host cell is a Enterobacteriaceae strain, optionally a Pantoea, Klebsiella, Erwinia or E. coli strain or a Pseudomonadacea strain.