Method and apparatus for producing a stablized antimicrobial non-toxic electrolyzed saline solution exhibiting potential as a therapeutic

1. A method for producing a stable, non-toxic, antimicrobial electrolyzed saline solution comprising:

preparing a saline solution having a saline concentration within a range from approximately 0.15% to approximately 1.0% by weight;

inserting within the saline solution an inert anode and a spaced apart corresponding inert cathode associated with a power source;

regulating the temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates and regulate relative concentrations of resulting components during electrolysis;

circulating the saline solution to maintain a flow of the saline solution between the anode and cathode;

applying an effective voltage potential selected from within a voltage range of zero volts to approximately thirty volts between the cathode and the anode sufficient to produce a mixture of chemically reduced and oxidized molecules, the mixture including hypochlorous acid, hypochlorites, dissolved oxygen chlorine, hydrogen gases, hydrogen peroxide, hydrogen, hypochloride and corresponding amounts of superoxides, ozone, activated hydrogen, chloride ions, hydroxides, singlet oxygen, and other forms of reactive oxygen species, wherein the total combination of hypochlorous acid, hypochlorites, and dissolved oxygen chlorine is at a concentration between 1 to 200 ppm, wherein the total combination of hydrogen gases, hydrogen peroxide, hydrogen, hypochloride, superoxides, and ozone is at a concentration from 1 to 50 ppm, wherein total reactive oxygen species in the balanced mixture is between 0.05 to 50 ppm; and

utilizing one or more techniques to achieve desired electrolysis efficiencies and stable specie compositions containing stable reactive oxygen species while preventing production of chlorates, the one or more techniques including one or more of the following:

electron and proton donation;

ion and dissolved-gas transport;

adjusting a temperature of the saline solution;

adjusting anode and cathode spacing;

adjusting a saline solution circulation rate; and

optimization of the effective voltage;

mirroring the mixture of chemically reduced and oxidized species in the electrolyzed saline solution to the reduced species and reactive oxygen species found in a known biological system by measuring concentrations of reactive oxygen species in the electrolyzed saline solution using a fluoro spectrometer and at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein; and

producing the stable, non-toxic, antimicrobial electrolyzed saline solution.

2. The method of claim 1 , wherein the step of regulating the temperature of the saline solution further comprises a step for maintaining a desired temperature of the saline solution between 30° F. and 100° F.

3. The method of claim 1 , wherein the effective voltage potential is less than thirty volts.

4. The method of claim 1 , further comprising a step for circulating the saline solution.

5. The method of claim 1 , wherein the inert cathode is positioned coaxially in relation to a position of the inert anode.

6. The method of claim 5 , wherein the inert cathode comprises a base metal selected from the group consisting of platinum, niobium, and titanium.

7. The method of claim 5 , further comprising a step for providing a spacing between a surface of the inert cathode and a surface of the inert anode, wherein the spacing less than or equal to one inch.

8. The method of claim 1 , further comprising a step for maintaining a pH of the saline solution between approximately 7.2 and approximately 7.5.

9. A method for producing an electrolyzed saline solution comprising:

electrolyzing a saline solution having a salt concentration between about 0.15% and about 1.0% by weight using a voltage between about 0 V and about 30 V across an inert anode and a spaced apart inert cathode thereby generating a target mixture of chemically reduced and oxidized molecules within the saline solution;

maintaining a solution temperature sufficient to prevent production of chlorates;

mirroring the target mixture of chemically reduced and oxidized species in the electrolyzed saline solution to the reduced species and reactive oxygen species found in a known biological system by measuring concentrations of reactive oxygen species in the electrolyzed saline solution using a fluorospectrometer and at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein; and

producing the electrolyzed saline solution.

10. The method of claim 9 , wherein the mixture includes hypochlorous acid, hypochlorites, dissolved oxygen chlorine, hydrogen gases, hydrogen peroxide, hydrogen, hypochloride and corresponding amounts of superoxides, ozone, activated hydrogen, chloride ions, hydroxides, singlet oxygen, and other forms of reactive oxygen species.

11. The method of claim 10 , wherein the total combination of hypochlorous acid, hypochlorites, and dissolved oxygen chlorine is at a concentration between 1 to 200 ppm, wherein the total combination of hydrogen gases, hydrogen peroxide, hydrogen, hypochloride, superoxides, and ozone is at a concentration from 1 to 50 ppm.

12. The method of claim 11 , wherein total reactive oxygen species in the mixture is between 0.05 to 50 ppm.

13. The method of claim 11 , wherein the electrolyzed saline solution is stable.

14. The method of claim 11 , wherein the electrolyzed saline solution is non-toxic.

15. The method of claim 11 , wherein the electrolyzed saline solution is antimicrobial.