Methods and compositions for targeting skip
Provided herein are methods and compositions for modulating apoptosis by targeting SKIP (Ski-interacting protein) activity. Methods of increasing DNA damage-induced cell death in cancer cells, and reducing DNA damage-induced cell death in normal cells are provided.
1. A method of treating colon cancer in an individual comprising: administering to the individual an effective amount of an anti-SKIP siRNA, thereby treating colon cancer in the individual.
2. The method of claim 1 , further comprising treating the individual with a DNA damaging agent.
3. The method of claim 2 , wherein treatment with the DNA damaging agent is before or concurrent with said administering.
4. The method of claim 2 , wherein the DNA damaging agent is a chemotherapeutic agent or radiotherapy.
5. The method of claim 1 , wherein the inhibitor of SKIP is flavopiridol.
6. A method of selectively inducing apoptosis in a colon cell comprising: contacting the colon cell with an anti-SKIP siRNA, thereby selectively inducing apoptosis in a colon cell.
7. The method of claim 6 , further comprising treating the colon cell with a DNA damaging agent.
8. The method of claim 7 , wherein treatment with the DNA damaging agent before or concurrently with the contacting.
9. The method of claim 6 , wherein the colon cell forms part of a colon tumor.
10. The method of claim 6 , wherein the inhibitor of SKIP is flavopiridol.
11. The method of claim 6 , wherein the colon cell is in an individual.
12. The method of claim 11 , wherein the individual has colon cancer.