L-cysteine producing bacterium and a method for producing L-cysteine
The present invention describes a bacterium belonging to the family Enterobacteriaceae which has L-cysteine-producing ability and has been modified to decrease the activity of a protein encoded by the d0191 gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, derivatives thereof, or a mixture thereof is collected from the medium.
1. A method for producing an L-amino acid selected from the group consisting of L-cysteine, L-cystine, derivatives thereof, and combinations thereof, which comprises culturing a bacterium belonging to the family Enterobacteriaceae in a medium and collecting the L-amino acid from the medium,
wherein the bacterium has L-cysteine-producing ability and has been modified to decrease the activity of a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO:2, and
(B) a protein comprising the amino acid sequence of SEQ ID NO:2 but which includes substitutions, deletions, insertions, or additions of 1 to 10 amino acid residues, and wherein said protein comprises cysteine desulfhydrase activity.
2. The method according to claim 1 , wherein the derivative of L-cysteine is a thiazolidine derivative.
3. The method according to claim 1 , wherein the activity of the protein is decreased by a method selected from the group consisting of decreasing the expression of a gene encoding the protein, and by disrupting the gene encoding the protein.
4. The method according to claim 1 , wherein the protein is encoded by a gene selected from the group consisting of:
(a) a DNA comprising the nucleotide sequence of SEQ ID NO:1, and
(b) a DNA which is able to hybridize with a sequence complementary to the nucleotide sequence of SEQ ID NO:1under stringent conditions comprising washing at 60° C., 0.1×SSC, 0.1% SDS, and encodes a protein comprising cysteine desulfhydrase activity.
5. The method according to claim 1 , wherein the bacterium further comprises a serine acetyltransferase which has been mutated so that feedback inhibition by L-cysteine is attenuated.
6. The method according to claim 1 , wherein the bacterium is a Pantoea bacterium.
7. The method according to claim 6 , wherein the bacterium is Pantoea ananatis .