Interleukin-1 alpha antibodies and methods of use
Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors.
1. A method of killing a cell expressing human interleukin-1alpha (IL-1α), the method comprising the step of contacting a mixture of the cell and human peripheral blood mononuclear cells with an amount of a purified human IgG1 monoclonal antibody (mAb) that specifically binds to human IL-1α, wherein the amount is sufficient to kill the cell and the cell is killed, and wherein the human IgG1 mAb comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:9 and a light chain comprising the amino acid sequence of SEQ ID NO:11.